[关键词]
[摘要]
目的 对准噶尔乌头中2种成分水溶液在不同加热条件下稳定性进行考察,通过液质联用方法测定其水解产物,分析其水解变化规律,并在准噶尔乌头药材中进行验证,分析准噶尔乌头炮制前后生物碱类成分的变化情况,总结其变化规律,揭示准噶尔乌头炮制水解机制,为准噶尔乌头炮制工艺参数的确定提供理论依据。方法 采用单一成分水溶液在不同加热条件下稳定性研究方法,以准噶尔乌头中2种主要化学成分乌头碱和脱氧乌头碱为研究对象,结合HPLC和HPLC-MS测定结果,总结各生物碱单体的变化规律,并在准噶尔乌头不同时间的炮制品中进行验证。结果 经过不同加热时间后,乌头碱及脱氧乌头碱的变化最为明显,乌头碱水溶液经过不同加热时间后主要生成一些水解产物,随着加热时间的增加,乌头碱逐渐减少,直至检测不到。结论 初步确定准噶尔乌头的炮制工艺参数为加热5~6 h,乌头碱及脱氧乌头碱发生水解产生了新的水解产物,从而达到减毒增效的效果。
[Key word]
[Abstract]
Objective To determinate the hydrolysis products of two ingredients in Aconitum soongaricum under different heating conditions by liquid chromatography-mass spectrometry method, analyze its hydrolysis change rule, and verify it in A. soongaricum, analyze the changes of alkaloid before and after processing, and summarize the change rules and processing hydrolysis mechanism, so as to provide theoretical basis for the processing parameters of A. soongaricum. Methods Taking two kinds of main chemical constituents in A. soongaricum, aconitine and deoxyaconitine as the research object, the change rule of alkaloid monomer variation under different heating conditions was summarized combined with HPLC and HPLC-MS results, which was verified in different processed products in A. soongaricum. Results There was the significant change in the content of aconitine and deoxyaconitine after different heating time, the content of the mainly products from aconitine hydrolysis was increased with the increase of heating time, and aconitine and deoxyaconitine were gradually disappeared, eventually not detected. Conclusion The processing parameters of A. soongaricum were preliminarily determined as heating for 5-6 h, and the hydrolysis of aconitine and deoxyaconitine producing new hydrolysis products, thus achieving the effect of reducing toxicity and increasing efficiency.
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[基金项目]
天山雪松计划:科技创新领军人才后备人选(2017XS12)