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[摘要]
目的 探究广西北部湾来源真菌Penicillium sp. H1中的活性天然产物。方法 利用柱色谱等手段对发酵产物进行分离、纯化,运用紫外、质谱、核磁共振和比旋光等方法鉴定化合物的结构,采用药敏纸片法评价化合物的抗香蕉枯萎病菌活性。结果 从真菌Penicillium sp. H1的发酵产物中分离获得了3个二萜类化合物,分别鉴定为炭角菌素D(1)、BE-31405(2)和sordaricin(3)。化合物1和2对香蕉枯萎病菌有中等的抑制活性,最小抑菌浓度(MIC)分别为32.0、16.0 μg/mL。结论 化合物1为未见文献报道的新的二萜糖苷类化合物。
[Key word]
[Abstract]
Objective To identify the active metabolites of Penicillium sp. H1 isolated from Beibu Gulf of Guangxi. Methods The isolation and purification of compounds were performed by means of column chromatography with silica gel. And their structures were elucidated through the analysis of UV, MS, NMR, and specific rotations. The bioactivities of compounds against Fusarium oxysporum f. sp. cubense were assayed by paper diffusion. Results Three diterpenes (1-3) were isolated from the fermentation broth of Penicillium sp. H1. Compound 1 was identified as a new compound and named xylarinonericin D. Compounds 2 and 3 were identified as BE-31405 (2) and sordaricin (3), respectively. Compounds 1 and 2 showed moderate antifungal activity against Fusarium oxysporum f. sp. cubense with MIC values of 32.0 μg/mL and 16.0 μg/mL, respectively. Conclusion Compound 1 is a new diterpene glycoside.
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[基金项目]
广西民族大学引进人才科研启动项目(2017KJQD004);2018年广西高校中青年教师基础能力提升项目(2018KY0177);广西科技基地和人才专项(2017AD19029);广西创新驱动发展重大专项子课题资助项目(桂科AA17204087-21)