[关键词]
[摘要]
目的 研究蔷薇科蔷薇属植物金樱子Rosa laevifgata根的化学成分。方法 采用硅胶、Sephadex LH-20、半制备高效液相等色谱技术进行分离纯化,根据理化性质和波谱数据鉴定化合物的结构,并进行体外抗炎活性实验。结果 从金樱子根的甲醇提取物中分离得到7个三萜类化合物,分别鉴定为18,19-裂环-2α,3β,23α-三羟基-19-羰基-乌苏烷-11,13(18)-二烯-28-羧酸(1)、(2R,19R)-甲基-2-乙酰氧-19-羟基-3-羰基-乌苏烷-12-烯-28-羧酸(2)、坡模酮酸(3)、号角树酸-3-甲酯(4)、2-乙酸基委陵菜酸(5)、坡模酸(6)、2α,3α-二羟基乌苏烷-12,18-二烯-28-羧酸(7)。结论 化合物1为1个新化合物,命名为金樱子裂环三萜A。体外抗炎活性实验结果表明,化合物1~7对脂多糖(LPS)刺激小鼠巨噬细胞(RAW264.7)释放NO具有明显的抑制作用,其中化合物1和7的抑制效果最为显著,显示了较好的体外抗炎活性。
[Key word]
[Abstract]
Objective Seven triterpenes were isolated from the methanol extract of the roots of Rosa laevifgata. Methods The compounds were isolated and purified by a combination of various chromatographic approaches, including silica gel, Sephadex LH-20, semipreparative HPLC and so on. Their structures were identified on the basis of pfysicochemiscal and spedtroscopic analysis. The in vitro anti-inflammatory activity test was investjgated. Results On the basis of spectroscopic data analysis, their structures were identified as 18,19-seco-2α,3β,23α-trihydoxyl-19-oxo-urs-11,13(18)-dien-28-oic acid (1), (2R,19R)-methyl 2-acetyloxy-19-hydroxyl-3-oxo-urs-12-en-28-oic acid (2), pomonic acid (3), cecropiacic acid 3-methyl ester (4), 2-acetyl tormentic acid (5), pomolic acid (6), and 2α,3α-dihydroxyurs-12,18-dien-28-oic acid (7). Conclusion Among them, compound 1 named as rosasecotriterpene A is a new triterpene. The anti-inflammatory activities of compounds 1-7 are tested in vitro. The results show that all the isolated compounds have obvious inhibitory effects on the release of NO from RAW264.7 cells induced by LPS. The inhibitory effects of compounds 1 and 7 are more significant, showing good anti-inflammatory activity in vitro.
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[基金项目]
湖南省土家医药研究中心开放项目(20172001);植物资源保护与利用湖南省高校重点实验室开放项目(JSK2017YX008);杜仲综合利用技术国家与地方联合工程实验室开放项目(NLE201705);湖南省重点学科建设项目(JSU0713)