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[摘要]
目的 探究天丝饮对慢性应激大鼠血清代谢组学的影响及作用机制。方法 采用去势后慢性应激制备大鼠模型,ig天丝饮3.5 g/kg,给药6周后进行水迷宫实验和核磁代谢组学检测。结果 水迷宫实验结果显示,与对照组比较,模型组大鼠潜伏距离延长(P<0.01),穿越平台次数减少(P<0.05);与模型组比较,给药组大鼠逃避潜伏距离缩短(P<0.01),穿越平台次数增加。核磁代谢组学结果显示模型组与对照组比较,代谢物丙氨酸、脲基乙内酰脲、精氨酸、肌酸、丙酮酸、丝氨酸含量增加,天冬酰胺酸、肉碱、甘油、N,N-二甲基甘氨酸、N-乙酰谷氨酰胺、苏氨酸、缬氨酸含量减少;葡萄糖、谷氨酰胺、甲基组氨酸含量在不同化学位移表现出不同的变化趋势。天丝饮组大鼠与模型组比较,代谢物精氨酸与丙氨酸均减少;天丝饮组大鼠与对照组比较,代谢物缬氨酸、精氨酸、谷氨酰胺、乙酰乙酸、天冬酰胺酸、赖氨酸、甘油、肉碱、2-氨基-3-羟基丁酸均减少,代谢物脲基乙内酰脲增多。结论 天丝饮对慢性应激大鼠血清代谢组学作用机制主要表现在调节氨基酸代谢。
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[Abstract]
Objective To explore the serum metabolomics mechanism of Tiansi Liquid on chronic stress rats. Methods The chronic unpredictable mild stress model with ovariectomization was utilized by giving Tiansi Liquid 3.5 g/kg, detection was carried out by Morris water maze and NMR metabolomics after six weeks of administration. Results The Morris water maze result showed that, compared with the control group, the distance to zone platform prolonged (P < 0.01) and the times of passing platform decreased (P < 0.05) in model group. While, the distance to zone platform shortened (P < 0.01) and the times of passing platform increased in treatment group compared with the model group. The NMR metabolomics showed that compared with the control group, the contents of alanine, allantoin, arginine, creatine, pyruvate, and serine were increased in model group, and the contents of asparagine, carnitine, glycerol, N,N-dimethylglycine, N-acetylglutamine, threonine, and valine were decreased in model group. The contents of glucose, glutamine, and methylhistidine in model group show different trends at different chemical shift. Compared with model group, the content of arginine and alanine were decreased in treatment group. Compared with the control group, the content of valine, arginine, glutamine, acetoacetate, asparagine, lysine, glycerol, carnitine, and threonine were decreased in treatment group, while the content of allantoin was increased in treatment group. Conclusion The main metabolomics effect of Tiansi Liquid on chronic stress rats was amino acid metabolism.
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[基金项目]
北京市自然科学基金资助项目(18G40092)