目的 分析鉴定口服补骨脂-肉豆蔻药对后大鼠血中移行成分，进一步研究补骨脂-肉豆蔻配伍对移行成分的影响。方法 采用超高效液相色谱-四极杆飞行质谱（UPLC-Q-TOF/MS）技术快速分析补骨脂-肉豆蔻药对提取物在大鼠体内移行成分，并结合Peakview软件进行分析。结果 与体外补骨脂提取物相比，有15个原型成分入血；与体外补骨脂-肉豆蔻药对提取物相比，有26个原型成分入血；与体外肉豆蔻药材提取物相比，有6个原型成分入血。结论 UPLC-Q-TOF/MS能快捷、准确、较全面地鉴定补骨脂-肉豆蔻药对的化学成分，为补骨脂-肉豆蔻药对的药效物质基础和质量控制等研究奠定了基础。

Objective To analyze and identify the transitional constituents of combination Psoralea corylifolia-Myristica fragrans in vivo and in vitro, and further study the effect of this combination on transitional components. Methods A rapid ultra-performance liquid chromatography/orthogonal acceleration time-of-flight mass spectrometry (UPLC-Q-TOF/MS) method was established to rapidly analyze constituents of before and after combination P. corylifolia-M. fragrans after oral administration in rats, and combined with Peakview software analysis. Results Compared with in vitro extract of P. corylifolia, there are 15 prototypes absorbed into the blood. Compared with in vitro combination P. corylifolia-M. fragrans extract, 26 prototype components were absorbed into the blood. Compared with M. fragrans extract, six prototype components were absorbed into the blood. Conclusion By using UPLC-Q-TOF/MS method, the main chemical constituents from the combination can be rapidly and accurately identified, and the results would facilitate the quality control of combination P. corylifolia-M. fragrans for safe and efficient use.

国家中医药重点学科临床中药学建设项目（国中医药人[2012]32号教发）；安徽中医药大学科学研究基金项目（2015fy003）；2016年度省卫生计生委中医药科研课题（2016zy80）