[关键词]
[摘要]
目的 研究红毛七Caulophyllum robustum的化学成分及其抗肿瘤活性。方法 采用硅胶柱色谱、葡聚糖凝胶Sephadex LH-20等色谱方法进行分离纯化,根据理化性质和波谱技术鉴定化合物的结构;采用CCK-8法,对化合物进行体外抑制人非小细胞肺癌A549细胞增殖的活性筛选。结果 从红毛七根及根茎中分离出11个化合物,分别鉴定为collinsogenin(1)、常春藤皂苷元(2)、eechinocystic acid 3-O-α-L-arabinopyranoside(3)、magnolamide(4)、木兰箭毒碱(5)、columbamine(6)、4,4'-diphenylmethane-bis(methyl)Carbamate(7)、(-)-8,8'-二甲氧基-开环异落叶松树脂酚-L-O-β-D-吡喃葡萄糖苷(8)、紫花松果菊苷A(9)、2-[4-(3-hydroxy-1-propenyl)-2-methoxyphenoxy]-1,3-propanediol(10)、亚油酸甘油酯(11)。结论 化合物1~2和4~11均首次从该属植物中分离得到;化合物1和2对人非小细胞肺癌A549细胞增殖具有中等强度的抑制作用。
[Key word]
[Abstract]
Objective To study the chemical constituents of Caulophyllum robustum and its antitumor activities. Methods The chemical constituents were isolated and purified with silica gel column chromatography, dextran gel Sephadex LH-20, and other chromatographic methods. Their structures were determined by physicochemical and spectral analysis. Furthermore, the cytotoxicity of these chemical components against the NSCLC A549 cell line was measured by CCK-8 method. Results Eleven compounds were isolated and identified as collinsogenin (1), hederagenin (2), eechinocystic acid 3-O-α-L-arabinopyranoside (3), magnolamide (4), (-)-magnocurarine (5), columbamine (6), 4,4'-diphenylmethane-bis (methyl) carbamate (7), (-)-8,8'-dimethoxy-L-O-(β-D-glucopyranosyl) secoisolariciresinol (8), asechipuroside A (9), 2-[4-(3-hydroxy-1-propenyl)-2-methoxyphenoxy]-1,3-propanediol (10), and 1-linoleoylglycerol (11). Conclusion Compounds 1-2 and 4-11 are isolated from Caulophyllum robustum genus for the first time. Compounds 1 and 2 show moderate inhibited effect on the proliferation of NSCLC A549 cell line.
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[基金项目]
国家科技支撑计划项目(2012BAI27B06);"重大新药创制"科技重大专项(2017ZX09301060);中南民族大学中央高校基本科研业务费专项(CZW17021)