目的 利用表面分子印迹技术制备硅胶表面高良姜素分子印迹聚合物（MIP）。方法 采用 (3-氨丙基) 三甲氧基硅烷对硅胶进行改性，并以改性硅胶为载体，高良姜素为模板分子，甲基丙烯酸（MAA）为功能单体，N,N'-亚甲基二丙烯酰胺（MBA）为交联剂，在优化实验条件下利用表面接枝聚合法合成高良姜素表面分子印迹聚合物（MIP）。采用红外光谱、扫描电镜对聚合物进行表征，静态吸附及竞争性吸附研究聚合物的吸附性能。结果 最佳制备条件为高良姜素与MAA的物质的量比为1∶4，MAA与MBA的物质的量比为1∶7，反应温度40℃，反应时间12 h。红外光谱和扫描电镜结果显示，印迹聚合物成功接枝到硅胶表面上并出现了对高良姜素分子产生选择性识别的孔穴和位点。吸附实验表明，该MIP对高良姜素分子具有特异的识别性和较好的亲和性，相对于对照物灯盏花素和木犀草素，MIP对高良姜素的选择性系数分别为11.2和5.3。结论 MIP对高良姜素识别性好、选择性强，为中药中黄酮类化合物的分离、提取提供了一种新方法。

Objective To prepare surface molecular imprinted polymers (MIP) of galangin by using surface molecular imprinting technique. Methods Galangin MIP was prepared by surface polymerization method at the surface of silica gel, which was modified with (3-aminopropyl) trimethoxysilane, by using galangin as the template molecule, methacrylic acid (MAA) as the functional monomer, and N,N'-methylenebisacrylamide (MBA) as crosslinking agent. The polymer was characterized by infrared spectroscopy and scanning electron microscopy. And its adsorption properties were studied by static and competitive adsorption method. Results The experimental research showed that the optimal preparation condition was that the molar ration of galangin to MAA was 1:4, with molar ration of MAA to MBA 1:7, reaction temperature 40℃ and reaction time 12 h. Infrared spectrum and scanning electron microscopy showed that MIP was successfully grafted on the surface of silica gel, and recognition holes and sites selectively appeared for galangin molecules. Adsorption experiments exhibited that MIP had specific recognition and good affinity for galangin molecules. Compared to the controls of breviscapine and luteolin, the selectivity coefficients of MIP to galangin were 11.2 and 5.3, respectively. Conclusion MIP has good recognition and high selectivity for galangin, which provides a new method for the separation and extraction of flavonoids from Chinese medicine.

山西省自然科学基金资助项目（2015011024）；山西医科大学青年基金资助项目（057620）