目的 研究有机溶液环境中阿魏酸的纳滤“强化”分离行为。方法 以阿魏酸为指标，采用Box-Behnken中心组合设计建立数学模型，考察截留相对分子质量、乙醇体积分数、溶液pH值对阿魏酸纳滤截留的影响，筛选乙醇体积分数与溶液pH敏感区域；采用纳滤传质数学模型拟合传质系数与有机溶剂浓度的相关性，分析有机溶剂对阿魏酸纳滤“强化”分离规律。结果 截留相对分子质量450纳滤膜，pH 8.0，乙醇体积分数由20%升高至40%，传质系数呈下降趋势，阿魏酸出现“强化”截留分离行为，在相同条件下，溶剂更换成甲醇和乙腈，也产生相同的“强化”截留分离效应，3种常见有机溶剂的体积分数与“强化”效应呈正相关，表现为乙醇≈甲醇>乙腈。结论 纳滤“强化”分离效应与有机溶剂的种类和体积分数相关，以阿魏酸为例探索有机溶液环境下的纳滤分离机制，为有机溶剂环境下中药中酚酸类成分的常温化富集提供理论支撑。

Objective To explore the enhanced separation behavior of ferulic acid in organic solution by nanofiltration. Methods In the experiment, molecular weight cut-off (MWCO) of nanofiltration membrane, ethanol concentration, and solution pH were selected as influencing factors to find the sensitive region of ethanol concentration and pH on the retention rate of ferulic acid with Box-Behnken central composite experiment design. And then, the separation rule of ferulic acid with organic solution was analyzed, the correlation between mass transfer coefficient and concentration of organic solvent was fitted with the changed organic solution by nanofiltration mass transfer mathematical model. Results Experiments indicated that the enhanced separation behavior appeared and the mass transfer coefficient decreased as the ethanol concentration increased from 20% to 40% with MWCO 450 and pH 8.0. Under the same condition, the enhanced separation behavior happened as the solvent was changed into methanol and acetonitrile, and the enhanced effect was positively correlated with the concentration of the three common organic solvents, the effect rule was ethanol ≈ methanol > acetonitrile material. Conclusion The enhanced separation effect of nanofiltration was related to the type and concentration of organic solvent. And taking ferulic acid as an example, the mechanism of nanofiltration separation in the environment of organic solution was studied, and the results provided references for nanofiltration concentrate for heat-sensitive traditional Chinese medicine of phenolic acid in organic solution.

国家自然科学基金资助项目（81503258，81603307）；江苏省高校自然科学基金项目（17KJB360010）；国家中药标准化行动计划项目（ZYBZH-C-JS-34）