目的 通过研究远志在不同光质光强下的生长、生理特性及成分变化情况，为远志的人工栽培，提高产量、增加目标成分含量提供理论依据。方法 以远志直播苗为实验材料，光质设置白光、黄光、红光、蓝光4种光质，光强设置100、300、500 μmol/(m²·s) 3个强度梯度，光照30 d后测定远志的株高、根长、叶长、叶宽、丙二醛（MDA）含量、抗氧化酶活性及主要活性成分含量。结果 红光下远志的株高、根长、叶长、生物量均达到最大。黄光下MDA含量最小，白光下MDA含量最大。红、蓝光下超氧化合物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性显著升高。蓝光下总黄酮、总酚、3,6'-二芥子酰基蔗糖含量最高。不同光质对远志𠮿酮Ⅲ的含量无显著性影响。300 μmol/(m²·s) 的光照强度下远志株高最高生物量最大，500 μmol/(m²·s) 光强下远志根长最长。叶片中的MDA含量及SOD、POD、CAT活性随着光照强度的增大而逐渐增大。不同光强对总黄酮、总酚及远志𠮿酮Ⅲ含量无显著性影响，300 μmol/(m²·s) 下3,6'-二芥子酰基蔗糖含量最高。结论 红光对远志根的促进作用最强，红、蓝光可显著提高POD、SOD、CAT活性，蓝光对总黄酮、总酚、3,6'-二芥子酰基蔗糖积累的促进最强。500 μmol/(m²·s) 的光强对远志根的促进作用最强，且叶片的POD、SOD、CAT活性较高，不同光强对总黄酮、总酚及远志𠮿酮Ⅲ含量无显著性影响，300 μmol/(m²·s) 可促进3,6'-二芥子酰基蔗糖的积累。

Objective To study the growth physiology and components of Polygala tenuifolia under different light quality and intensity, and provide theoretical basis for the artificial cultivation, yield improvement, and increasing the target composition of P. tenuifolia. Methods Using P. tenuifolia shoots as the experimental material, four kinds of ights (white, yellow, red, and blue) as light quality, the light intensity was set at 100, 300, and 500 μmol/(m²·s) to determinate the plant height, root length, leaf length, leaf width, malondialdehyde (MDA) content, antioxidant enzyme activity, and secondary metabolite content in P. tenuifolia after 30 d of illumination. Results Under the red light, P. tenuifolia had the highest plant height, the longest root length and leaf length, and the largest biomass. Under the yellow light, the MDA content was the lowest, and the MDA content was the highest under white light. The activities of SOD, POD, and CAT were significantly increased under red and blue light. The highest content of total flavonoids, total phenols, and 3,6'-disinapoyl sucrose under blue light. Different light qualities had no significant effect on the content of polygalaxanthone Ⅲ. The biomass of plant height and the 3,6'-disinapoyl sucrose content were the highest under the light intensity of 300 μmol/(m²·s). P. tenuifolia had the longest roots under the light intensity of 500 μmol/(m²·s), and the content of MDA and the activities of POD, SOD, and CAT increased with the increase of light intensity. Conclusion Red light has the strongest promoting effect on the roots of P. tenuifolia. Red and blue light significantly increases the activity of POD, SOD, and CAT. Blue light promotes the accumulation of total flavonoids, total phenols, and 3,6'-disinapoyl sucrose. The light intensity of 500 μmol/(m²·s) has the strongest promoting effect on the roots of P. tenuifolia, and the activities of POD, SOD, and CAT in leaves were higher. Different light qualities had no significant effect on the contents of total flavonoids, total phenols, and polygalaxanthone Ⅲ. The light intensity of 300 μmol/(m²·s) promotes the accumulation of 3,6'-disinapoyl sucrose.

公益性行业（中医药）科研专项经费项目（201507002-1-08）；国家大学生创新计划（201710716011）