目的 获得白鲜Dictamnus dasycarpus根转录组信息特征。方法 以白鲜根为研究对象，采用Illumina HiSeq^TM 2000 150PE进行高通量转录组测序并进行数据分析。结果 转录组测序共获得69 643 286条高质量序列（clean reads），Trinity de novo组装获得49 050条unigenes，平均长度841 nt。BLAST分析显示分别有31 636（64.49%）、22 367（45.60%）、19 246（39.23%）、12 595（25.68%）条unigenes在NR、Swiss-port、KOG、KEGG数据库得到注释信息，可归为GO分类的生物过程、细胞组分和分子功能3大类42分支，涉及132个KEGG标准代谢通路，其中包括18个次生代谢标准通路。进一步分析获得90个基因参与多种生物碱生物合成通路。蛋白编码框序列1 908个，高等植物转录因子55个家族；借助MISA软件发现4 579个SSRs，三碱基重复最丰富，有2 021个，出现频率为44.1%；五碱基重复SSRs仅占3.5%。结论 利用高通量测序技术获得白鲜根转录组信息特征，为后期白鲜基因功能鉴定、次生代谢途径解析及其调控机制研究奠定基础。

Objective To obtain the transcriptome dataset of roots of Dictamnus dasycarpus. Methods The root transcriptome dataset of D. dasycarpus was obtained using the high-throughput sequencing platform Illumina HiSeq^TM 2000 150PE, followed by systemic bioinformatics analyses. Results A great number of 69 643 286 high quality clean reads were obtained by the transcriptome sequencing analyses. Using Trinity de novo assembling, a total of 49 050 unigenes were finally obtained, with an average length of 841 nt. BLAST analysis indicated that 31 636 (accounting 64.49% of the total unigenes), 22 367 (45.60%), 19 246 (39.23%), 12 595 (25.68%) unigenes were successfully annotated in the NR, Swiss-port, KOG, and KEGG databases, respectively. And GO classification contained the basic three major groups, including biological process, cellular component, and molecular function with 42 subgroups. A total of 132 KEGG standard metabolic pathways were designated, 18 of which were defied as the secondary metabolism. Further analysis revealed that a total of 90 unigenes were involved in the biosynthesis of various alkaloids. Of all unigenes, 1 908 were predicted to have CDS, and 55 families of plant transcription factors were also identified. Using MISA prediction, 4 579 simple sequence repeats (SSRs) were obtained, among which the tri-nucleotide SSRs were abundant with 2 021 (44.1%), whereas the penta-nucleotide SSRs accounted for 3.5%. Conclusion The root transcriptome of D. dasycarpus revealed by the high-throughput sequencing technology will be important for gene functional characterization, secondary metabolism pathway exploration, and regulation mechanism research in this species.

陕西省教育厅专项（18JK0216）；陕西中医药大学新进博士科研启动经费（104080001）；陕西省教育厅科学研究项目（17JK0215）；陕西省高校青年杰出人才支持计划项目；陕西省科技厅社发攻关项目（2013K14-03-08）；咸阳市中青年科技领军人才项目