目的 研究不同产地10批次大叶冬青药材的HPLC指纹图谱与其黄嘌呤氧化酶（XOD）抑制作用的谱效关系，初步揭示大叶冬青药材的药效物质基础。方法 HPLC法测定了10批次不同产地的大叶冬青药材样品，采用中药色谱指纹图谱相似度评价系统2004A版评价，建立共有模式，并进行主成分分析。采用HPLC法建立酶-底物反应体系，对不同批次的大叶冬青药材的XOD抑制活性进行研究，基于谱效关系，采用SPSS软件对数据进行相关性分析。采用氧嗪酸钾诱导建立小鼠高尿酸血症模型，检测小鼠血清中以及肝组织中尿酸（UC）、XOD水平变化。结果 10批次不同产地的大叶冬青药材HPLC指纹图谱中共有15个特征峰，其中峰11（苦丁茶冬青皂苷E）、14（苦丁冬青皂苷F）与XOD抑制作用呈中等程度相关，峰5（未鉴定）、6（山柰酚-3-O-β-D-芸香糖苷）、7（异鼠李素-3-O-β-D-芸香糖苷）、10（大叶冬青皂苷C）、13（大叶冬青皂苷D）相关性最强。由主成分分析可知，峰9（大叶冬青皂苷H）的量在大叶冬青药材质量控制中起到至关重要的作用。体内实验结果表明，大叶冬青提取物高、中、低剂量组均能显著降低小鼠血清UC和XOD水平，抑制肝组织中XOD活性，并呈现一定的量效关系。结论 10批不同产地的大叶冬青药材对XOD均有较强的抑制作用，HPLC指纹图谱显示出7个共有峰对应的化合物与该药效存在一定的相关性。

Objective To investigate the spectrum-activity relationship between HPLC fingerprint of Ilex latifolia from 10 batches in different regions and its inhibition of xanthine oxidase (XOD) efficacy and to reveal the material basis of I. latifolia. Methods The samples from 10 batch different regions were determined with the HPLC method. The similarity of the representative standard fingerprint was evaluated using the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (Version 2004A). The common pattern was established for the principal component analysis (PCA). The HPLC method was used to establish the enzyme-substrate reaction system, and the inhibitory activity of XOD of 10 batches of I. latifolia produced in different areas was studied. The SPSS analysis correlation was used to conduct correlation analysis based on spectrum-activity relationship. The model of mouse hyperuricemia were induced by potassium oxonate, and the levels of UC and XOD in plasma and liver were detected. Results Fifteen common peaks in HPLC-fingerprint of I. latifolia were obtained. It was tentatively concluded that peak 11 (ilekudinoside E) and peak 14 (kudinoside F) had the moderate correlation with the inhibitory effect of XOD in the fifteen characteristic peaks. Peaks 5, 6 (kaempferol-3-O-β-D-rutinoside), 7 (isorhamnetin-3-O-β-D-rutinoside), 10 (latifoloside C), and 13 (latifoloside D) had the strongest correlation. The quality of I. latifolia was influenced by the concentration of latifoloside H (peak 9) obtained by PCA. In the vivo experiment of hyperuricemia model mice, the levels of plasma UC and XOD of the mice in different doses of I. latifolia extracts groups were reduced, and the activities of XOD in livers tissue were also reduced. Conclusion Ten batches of samples from different habitats had potent inhibitory effects on XOD. There may be a certain relationship between seven compounds showed in HPLC fingerprint and its inhibitory effect on XOD.

吉林省科技发展计划项目（20150311037YY）；吉林省卫生计生科研计划项目（2014Z206）；吉林省中医药科技项目（2014-ZD28）