目的 应用星点设计-响应面法优化秦皮产地加工与饮片炮制一体化工艺。方法 以HPLC法和紫外分光光度法测得秦皮中秦皮甲素、秦皮乙素、秦皮素、秦皮苷和总香豆素含量的总评归一值（Y）为评价指标，以切制规格（X₁）、干燥温度（X₂）和干燥时间（X₃）为考察对象，利用星点设计效应面法优化秦皮的产地加工与饮片一体化工艺；并研究一体化秦皮饮片的抗炎镇痛效果。结果 最佳一体化工艺为秦皮药材去粗皮后，切6 mm厚的丝，75℃干燥3.25 h；该工艺生产的秦皮饮片可显著减少冰醋酸致小鼠的扭体反应次数，延长潜伏期，抑制角叉菜胶诱导的大鼠足肿胀反应，明显或部分缓解肿胀程度；一体化秦皮饮片比传统饮片镇痛作用更加明显，差异显著（P<0.05）。结论 响应面法优选秦皮产地加工一体化工艺简便易行，准确度较好，为其他皮类饮片生产提供参考，具有一定的推广应用价值。

Objective To optimize the processing integration technology of Fraxini Cortex based on response surface methodology. Methods The single factor experiment combined with Box-Behnken design was used to optimize the integrative technology, with five major characteristic components (aesculin, aesculetin, fraxetin, fraxin, and total coumarin) as indexes, in order to detect three factors (cutting thickness, drying temperature, and drying time), and research the effect of analgesic and anti-inflammatory of processing integration Fraxini Cortex. Results Optimum integrative technology of primary processing was as follows:cutting 6 mm Fraxini Cortex, and drying for 3.25 h at 75℃. The processing integration Fraxini Cortex significantly decreased the times of wrinkle reaction induced by acetic acid in mice, prolonged the latent period, and obviously or partially inhibited the feet swelling degree induced by carrageenan in rats. The analgesic effect of integrated technology group was more obvious than traditional technology group (P < 0.05). Conclusion This optimized integrative technology of Fraxini Cortex is reasonable and feasible with high accuracy. It could provide the scientific basis and innovative idea to the large-scale production of decoction pieces of Chinese materia medica and have the certain value for its promotion and application.

国家中医药管理局中医药行业科研专项（201507002）