目的 研究半夏总有机酸量与止咳作用、总有机酸HPLC指纹图谱与止咳作用、总有机酸量与止咳效价的相关性，建立物质基础-功效-生物活性多元化的半夏质量评价模式。方法 通过电位滴定法测定半夏总有机酸含量，采用HPLC建立总有机酸指纹图谱；以浓氨水引咳模型研究止咳作用，并利用灰色关联度分析法建立总有机酸与止咳作用的谱-效关系；基于小鼠浓氨水引咳模型，建立半夏止咳效价的测定方法；通过SPSS 18.0软件分析总有机酸和止咳作用、止咳效价3者之间的相关性。结果 半夏总有机酸对浓氨水致小鼠咳嗽有显著的抑制作用，结合总有机酸含量，随其含量的增加，止咳作用增强。在总有机酸HPLC指纹图谱中，4、6（琥珀酸）、12号色谱峰所代表的化学成分与止咳作用具有较强的相关性，对止咳作用贡献较大。10批半夏的止咳平均效价值为（753.72±58.18）U/g，结合总有机酸定量分析，二者呈显著的正相关，表现为总有机酸的量增加，止咳效价值增大。结论 总有机酸为半夏止咳作用的物质基础之一；总有机酸、止咳作用、生物活性3者之间呈相关性，构建了物质基础-功效-生物活性多元化的半夏质量评价模式。

Objective To establish the quality evaluation model of material foundation-efficacy-bioactivity of Pinelliae Rhizoma by investigating the relationships between the total organic acids of Pinelliae Rhizoma and the antitussive effect, the HPLC fingerprint of total organic acid and the antitussive effect, the total organic acids and the antitussive potency, respectively. Methods The content of total organic acids in Pinelliae Rhizoma was determined by potentiometric titration. The total organic acid fingerprints were established by HPLC. The cough relieving effect was studied by the concentrated ammonia water cough model, and the spectral-effect relationship between total organic acids and antitussive effect was established by the grey correlation analysis. Based on the mice concentrated ammonia water cough model, the determination method of the cough potency of Pinelliae Rhizoma was established. The correlation among the total organic acids, the antitussive effect and the antitussive potency was analyzed by SPSS 18.0 Software. Results The total organic acids of Pinelliae Rhizoma had a significant inhibitory effect on mice cough induced by concentrated ammonia. The antitussive effect was enhanced with the increase of the content of total organic acids. In the HPLC chromatogram, the chemical components represented by chromatographic peaks of 4, 6 (succinic acid), and 12 showed a strong correlation with the antitussive effect. The cough efficacy mean value of ten batches of Pinelliae Rhizoma was (753.72±58.18) U/g. Combined with the quantitative analysis of total organic acids, there was a significant positive correlation between them, showing an increase in the antitussive efficacy with an increase of the content of total organic acids. Conclusion This study not only revealed the material foundation of the anti-tussive effect of Pinelliae Rhizoma, but also clarified the correlation among total organic acids, antitussive effect and bioactivity. Finally, it can improve the quality standards of Pinelliae Rhizoma and provide a scientific basis for the construction of a new model for quality control of Pinelliae Rhizoma.

国家自然科学基金资助项目（81173492）；国家中药标准行动计划项目"川贝母、半夏等7种中药饮片标准化建设"（ZYBZH-Y-SC-40）；四川省科技厅科技成果转化项目"半夏优质高产栽培及绿色加工技术成果转化"（2017CC0084）；半夏优质高产栽培技术集成及产业化示范（2050205）；"川蓬1号""川半夏1号"新品种成果转化（17CZ0013）；金川县、丹巴县中药材科技扶贫示范基地建设（2017NFP0001）；川产道地药材半夏新品种"川半夏1号"推广与示范（2016NFP0076）