[关键词]
[摘要]
目的 对蒙药孟根乌苏中HgS微-纳米颗粒的提取、分离及体外溶出进行研究。方法 采用索氏提取法去除单质硫,确定孟根乌苏提取的最佳时间。采用差速离心法分离HgS颗粒;通过控制PVP浓度、投料比、离心机转速等因素获得不同粒径的HgS微-纳米颗粒;利用扫描电镜(SEM)及X-射线衍射法(XRD)分析颗粒形貌和结构组成;采用双硫腙比色法对比研究不同粒径范围的HgS颗粒、孟根乌苏原药材及孟根乌苏-18味丸在模拟人工胃液、人工肠液中的溶出情况。结果 分离得到的6个HgS颗粒样品的粒径分布较均匀,粒径分别为2 000~4 000、800~2 000、200~800、80~200、50~80、20~50 nm。提取后样品中主要成分为α-HgS及β-HgS。体外溶出结果表明,随着HgS颗粒粒径变小,汞的溶出度增大,表明汞的溶出与HgS颗粒粒径大小呈反比关系。同时汞的溶出度也受硫、pH值及复方的影响。结论 该法可简单、高效地分离HgS颗粒。为不同粒径HgS微-纳米颗粒的药动学和药理学研究提供了基础。
[Key word]
[Abstract]
Objective To study the extraction, separation, and in vitro dissolution method of HgS micro & nano-particles from Mongolian medicine Menken Usu. Methods The Soxhlet extraction method was used to investigate the sulfur from Menken Usu effectively, and the optimum time of extraction was determined by investigating the influence of different extraction time on the extraction rate of sulfur in the Menken Usu. Then, the mercuric sulfide particles were separated by differential centrifugation. The mercuric sulfide micro & nano-particles with different particle sizes can be obtained by controlling the concentration of PVP, molar ratio, centrifuge speed, and other factors. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) were used to analyze the morphology and composition of the particles. Using dithizone colorimetric method to determine and compare the dissolution of HgS particles with different sizes, Menken Usu original herbs, and Menken Usu-18 in simulated human gastric and small intestinal. Results The SEM results showed that the obtained six mercuric sulfide particle samples have a uniform size distribution:Uniform size were 2 000-4 000, 800-2 000, 200-800, 80-200, 50-80, and 20-50 nm. The main components in the extracted samples were α-HgS and β-HgS. As the particle size of HgS gradually became smaller, the dissolution of mercury was increased, which indicated that the size of HgS particle was inversely related to the dissolution of mercury. At the same time, the dissolution of mercury is also affected by sulfur, pH, and compounds.Conclusion This is a simple and efficient method for the separation of mercuric sulfide particles, and provides the basis for the pharmacokinetics and pharmacological study of mercuric sulfide nanoparticles with different particle size.
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[基金项目]
国家自然科学基金资助项目(81503351,81760750)