在前期提出的超分子“印迹模板”理论基础上，分析总结了中药四性的历史沿革与研究现状，运用超分子化学理论对中药四性的产生进行了深入剖析：人体与中药为具有相同“印迹模板”的巨复超分子体系，不同类型（热、寒性）中药有效成分群（客体分子）与不同状态下人体（主体分子）以非共价键发生结合，通过释放（或吸收）能量，能够纠正人体由于受到外界寒邪/热邪侵袭而造成机体超分子自识别、自组织、自组装、自复制过程发生偏移的情况，使机体回归正常状态，宏观上表现为中药四性。基于此，提出了中药四性研究的体内、体外两类方法，体外方法包括物理内能法和主客体分离及制备超分子聚合物的方法；体内方法包括生物体热研究、动物行为学观测法、体表温度观测法。超分子“印迹模板”理论是研究中药四性的有效工具，而超分子化学理论与传统中医药理论的融合必将成为推动传统中医药走向现代化的强大助力。

Based on the previous theory of supramolecular "imprinting template", the historical evolution and research status together with the generation of four properties of Chinese materia medica (CMM) were analyzed and summarized by using supramolecular chemistry theory:Human and Chinese medicine are giant complex supramolecular systems with the same "imprinting template". Different types (heat and cold) of active ingredients (guest molecules) of CMM and humans (host molecules) in different states bonded by non-covalent, releasing (or absorbing) energy, which can correct the self-recognition, self-organization, self-assembly, and self-replication of the body's basic supramolecular attacked by cold or heat, in order to make the body back to normal, in short the macroscopic manifestation was four properties of CMM. Thus, two methods were proposed for four properties of CMM including in vivo and in vitro ones, the former one includes physical internal energy method and host-guest separation and supramolecular polymer preparation; In vivo method includes biological thermal studies, animal behavioral observations, and body surface temperature observations. The supramolecular "imprinting template" theory is a key for studying four properties of CMM,the combination between supramolecular chemistry theory and traditional Chinese medicine theory will certainly become a powerful method to promote the modernization of CMM.

国家自然科学基金资助项目（81573691）；湖南省自然科学基金资助项目（2017JJ3236，2016JJ4065）；湖南省教育厅基金项目（17B200）；药学湖南省重点学科开放基金；中药成药性与制剂制备湖南省重点实验室开放基金；2011协同创新中心资助