目的 探讨解毒祛瘀滋阴方冻干粉与含药血清对脂多糖（LPS）诱导的小鼠单核巨噬细胞RAW264.7炎症信号通路的影响。方法 体外培养RAW264.7细胞，随机分为对照组、LPS组、解毒祛瘀滋阴方含药血清组、冻干粉组、LPS加含药血清组及LPS加冻干粉组。干预24 h后，采用CCK-8法筛选含药血清和冻干粉的最佳浓度并检测二者对细胞活力的影响；采用ELISA法测定细胞上清液中肿瘤坏死因子-α（TNF-α）含量；采用qRT-PCR法检测核转录因子-κB（NF-κB）、TNF-α mRNA的表达；采用Western blotting法检测NF-κB蛋白表达水平；采用LC-MS检测解毒祛瘀滋阴方含药血清中的有效成分。结果 与对照组比较，LPS刺激组TNF-α炎性因子水平、NF-κB、TNF-α mRNA表达水平和NF-κB蛋白表达水平显著升高（P<0.05），含药血清组TNF-α炎性因子水平、NF-κB、TNF-α mRNA表达水平和NF-κB蛋白表达水平较冻干粉组降低明显（P<0.05）；与LPS组比较，LPS加含药血清组TNF-α炎性因子水平、NF-κB、TNF-α mRNA表达水平和NF-κB蛋白表达水平降低较LPS加冻干粉组明显（P<0.05）；解毒祛瘀滋阴方含药血清中检测到芍药苷和阿魏酸。结论 解毒祛瘀滋阴方冻干粉和含药血清均具有抑制炎症信号通路的作用。

Objective To study the effects of Jiedu Quyu Ziyin Prescription (JQZP)-treated freeze dried powder and drug-containing serum on the inflammatory signal pathway of monocyte-macrophage induced by LPS (lipopolysaccharide) in mice. Methods Monocyte-macrophage cells were cultured in vitro and randomly divided into blank group, LPS stimulation group, drug-containing serum group, freeze dried powder group, LPS + drug-containing serum group, and LPS + freeze dried powder group. After 24 h intervention, the optimal concentrations of drug-containing serum and freeze dried powder were screened by CCK8 method and the cell viability was measured respectively. The content of tumor necrosis factor (TNF-α) in cell serum was measured by ELISA. Real-time PCR was employed to test the expression of TNF-α mRNA and nuclear transcription factor kappa-light-chain-enhancer of activated B cells (NF-κB). Western-blot was used to detect the expression of NF-κB protein. The LC-MS was used to detect the active ingredients in the drug-containing serum. Results Compared with the blank group, the expression of TNF-α level, NF-κB, TNF-α mRNA and NF-κB protein in LPS stimulation group were significantly increased (P < 0.05). Compared with the LPS stimulation group, the TNF-α level, NF-κB, TNF-α mRNA and the expression of NF-κB protein in the LPS plus serum group were significantly lower than those in the LPS plus freeze-dried powder group (P < 0.05). Paeoniflorin and ferulic acid were detected in the drug-containing serum. Conclusion JQZP freeze-dried powder and drug-containing serum all have the effect of inhibiting the inflammatory signaling pathway.

国家自然科学基金面上项目（81673863）；浙江省自然科学基金资助项目（LY15H270007）