目的 基于网络药理学和化学物质组学探讨灯台叶片生物碱的抗炎作用机制。方法 采用UPLC-Q/TOF解析灯台叶片中的主要吲哚类生物碱，通过网络药理学、反向对接以及生物信息学分析推测主要作用靶点和通路，并采用Western blotting方法对脂多糖（LPS）诱导的BEAS-2B细胞炎症模型主要节点蛋白的磷酸化水平进行验证。结果 灯台叶片提取物中主要含有的12种吲哚类生物碱，分别作用于PDPK1（PDK1）、MAPK1（ERK2）、MAPK8（JNK1）等多个炎症靶点，灯台叶片提取物能够降低LPS诱导的ERK2、JNK1、IKK的磷酸化水平。结论 灯台叶片中的吲哚类生物碱作为其抗炎的质量标志物，抑制了PI3K/Akt/NF-κB和MAPK通路，是其治疗慢性支气管炎潜在的分子机制。

Objective To investigate the anti-inflammatory mechanism of the alkaloids in Dengtaiye Tablets (DTYT) based on the network pharmacology and chemomics. Methods The main indole alkaloids in the DTYT from Folium Alstoniae Scholaris were identified by UPLC-Q/TOF. The network pharmacology coupled with virtual docking technology and bioinformatics analysis were carried out for screening the key protein targets and pathways. Western blotting was utilized for evaluating the phosphorylation level of the primary node proteins in BEAS-2B cells inflammatory model induced by LPS. Results Twelve indole alkaloids were found in DTYT extract, which act on different protein targets, such as PDPK1 (PDK1), MAPK1 (ERK2), and MAPK8 (JNK1) respectively, and reduce the level of ERK2, JNK1, and IKK phosphorylation induced by LPS. Conclusion As the Q-markers of anti-inflammatory, the indole alkaloids contained in DTYT inhibited the PDK1/AKT/NF-κB and MAPK pathways, which present potential molecular mechanisms for the treatment of chronic bronchitis of DTYT.