目的 探究五龙消癥丸对胃癌BGC-823细胞侵袭与迁移能力的影响及其作用机制。方法 分别用五龙消癥丸、PI3K激动剂胰岛素样生长因子1（IGF-1）及PI3K特异性抑制剂LY294002作用于BGC-823细胞，采用MTT法检测不同质量浓度五龙消癥丸对BGC-823细胞增殖的影响；Transwell小室模型研究五龙消癥丸对BGC-823细胞垂直侵袭和迁移能力的影响；采用ELISA法检测五龙消癥丸对血管内皮生长因子（VEGF）、基质金属蛋白酶2（MMP-2）和MMP-9表达的影响；采用Western blotting及RT-PCR法检测脂酰肌醇3-激酶（PI3K）/蛋白激酶B（Akt）/核转录因子-κB（NF-κB）信号通路中相关蛋白及mRNA的表达。结果 五龙消癥丸可显著抑制BGC-823细胞的增殖、黏附、侵袭和迁移能力，显著抑制BGC-823细胞中VEGF、MMP-2和MMP-9蛋白的表达，显著抑制p-PI3K、p-Akt、磷酸化IkB激酶-α（p-IKK-α）、p-NF-κB p65^{Ser 276}蛋白及PI3K、Akt、IKKα、NF-κB mRNA的表达，并且呈现一定的时效和量效关系。结论 五龙消癥丸通过抑制PI3K/Akt/NF-κB信号通路相关蛋白及mRNA表达，从而达到抑制BGC-823细胞的增殖、迁移、侵袭作用。

Objective To investigate the effect of Wulong Xiaozheng Pill (WXP) on the migration and invasion of human gastric cancer cell-line BGC-823 and its mechanism. Methods WXP, IGF-1, and LY294002 were added on BGC-823 cells. Then the inhibitory effect of WXP was detected by MTT assay. Transwell assay was performed to determine the migration and invasion capacity of on BGC-823 cells. Expressions of VEGF, MMP-2, and MMP-9 were detected by ELISA, while the expressions of related proteins and mRNA in PI3K/NF-κB signaling pathway were detected by Western blotting and RT-PCR. Results WXP can inhibit the proliferation, adhesion, invasion, and migration of BGC-823 cells. In addition, WXP inhibited the expression of VEGF, MMP-2 and MMP-9 protein in BGC-823 cells. WXP significantly inhibited the expression of p-PI3K, p-Akt, p-IKK-a, and p-NF-κB p65^{Ser 276} proteins, PI3K, Akt, IKKa, and NF-κB mRNA, which showed a time-dependent and dose-dependent manner. Conclusion WXP inhibit the capacity, migration and invasion of BGC-823 cells by blocking PI3K/NF-κB signaling pathway.

国家中医药管理局中医药科学技术研究专项（国中医药科2016ZX05）