目的 对ig给予具有拟雌激素活性的菟丝子醇提物后大鼠的体内化学成分进行定性分析。方法 应用HPLC-Q/TOF MS/MS技术，对大鼠尿液中的原型成分及代谢产物进行解析。结果 在含药尿液中表征出14个化学成分，鉴定出12个化学成分，其中包括6个原型成分，分别为山柰酚-3-β-D-葡萄糖醛酸苷、6-O-（反式）对香豆酰基-β-D-呋喃果糖-（2→1）-α-D-吡喃葡萄糖苷、山柰酚-7-鼠李糖苷、绿原酸、芹菜素、槲皮苷；6个代谢成分，分别为对羟基桂皮酸、对羟基苯丙酸、异鼠李素、山柰酚-3-O-葡萄糖苷、乙酰化咖啡酸、槲皮素硫酸酯。结论 应用HPLC-Q/TOF MS/MS技术能够简单、快速地分析大鼠ig菟丝子醇提物后尿液中的原型成分及代谢产物，为进一步阐明其药效物质基础提供了依据。

Objective To qualitatively analyze the chemical constituents in rats after intragastric administration of estrogenically active ethanol extract of Cuscuta chinensis. Methods The high performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF MS/MS) was used to identify the prototypes and metabolites in rat urine. Results Twelve chemical constituents were identified in the drug-containing urine, including six prototypes and six metabolites. The prototypes are kaemoferol-3-β-D-glucuronide, 6-O-(E)-p-coumaroyl-β-D-fructofuranosyl-(2→1)-α-D-glucopyranoside, aempferol-7-rhamnoside, chlorogenic acid, and apigenin. The metabolites are p-hydroxycinnamic acid, p-hydroxyphenylpropionic acid, isorhamnetin, kaempferol-3-O-glucoside, acetyl caffeic acid, and quercetin sulfate. Conclusion The method of HPLC-Q/TOF MS/MS is simple and rapid for the analysis of prototype components and metabolites in rats urine after oral administration of ethanol extract of C. chinensis, providing the basis for further clarification of the estrogen material basis of C. chinensis.

黑龙江省自然科学基金重点项目（ZD2017014）