目的 利用超高液相色谱-串联四极杆飞行时间质谱（UPLC-Q-TOF/MS）联用技术对北青龙衣大鼠肾组织化学成分进行分析。方法 大鼠ig给予北青龙衣醇提物后，采集肾组织，采用Waters Acquity UPLC BEH C₁₈色谱柱（100 mm×2.1 mm，1.7 μm），流动相为0.1%甲酸的水溶液（A）-0.1%甲酸的乙腈溶液（B），梯度洗脱，质谱采用电喷雾（ESI）离子源，在正离子模式下采集数据，通过Peakview 2.0/masterview 1.0和Metabolitepilot数据分析软件，通过对比保留时间、碎片丰度比、母离子精确质量数及二级质谱图鉴定和表征北青龙衣在大鼠肾组织成分。结果 从大鼠肾组织中鉴定了24种化学成分，包括16个原型成分和8个代谢产物，其中包含12个萘醌类、5个黄酮类、3个二芳基庚烷类、4个三萜类成分。结论 初步确定了北青龙衣在大鼠肾组织中原型成分及代谢产物，为北青龙衣用药安全性和有效性提供参考，为其在大鼠体内成分及组织分布研究提供方法学借鉴。

Objective To analyze the chemical constituents from rat kidney tissue of Juglans mandshurica by UPLC-Q-TOF/MS. Methods The kidney tissue was collected after oral administration of ethanol extract of J. mandshurica. The gradient elution was performed using a Waters Acquity UPLC BEH C₁₈ column (100 mm×2.1 mm, 1.7 μm), 0.1% formic acid water (A), and 0.1% formic acid acetonitrile (B) mobile phase system. An electrospray (ESI) ion source was used for mass spectrometry to collect data in positive ion mode. Combined with Peakview 2.0/masterview 1.0 and Metabolitepilot data analysis software, the kidney tissue components of J. mandshurica were identified by comparing the retention time, isotope kurtosis ratio, exact mass of the parent ion, and MS/MS fragment. Results Twenty-four chemical constituents including 16 prototypic components and eight metabolites were identified from rat kidney tissue, which contains 12 naphthoquinones, five flavonoids, three diarylheptanoids, and four triterpenoids. Conclusion The prototype components and metabolites of kidney tissue of J. mandshurica were determined. In further study, it provides reference for the safety and effectiveness of the treatment of J. mandshurica and provides a methodological reference for its in vivo composition and tissue distribution.

国家自然科学基金项目（81503348）；黑龙江省自然科学基金项目（H2017044）；国家中医药管理局中医药科学技术研究专项（2015-14）