[关键词]
[摘要]
目的 研究柳蒿Artemisia integrifolia的化学成分。方法 采用溶剂萃取、硅胶柱色谱、高效液相色谱等方法进行分离纯化,通过核磁共振谱等光谱数据分析鉴定结构;采用MTT法分别测定化合物对人宫颈癌HeLa细胞体外增殖抑制活性。结果 从柳蒿中分离得到10个倍半萜类化合物,分别鉴定为3-methoxy-tanapartholide(1)、dehydroleucodin(2)、4β-羟基愈创木-2,10(14),11(13)-三烯-12,6α-内酯(3)、4α-羟基愈创木-2,10(14),11(13)-三烯-12,6α-内酯(4)、2β-羟基愈创木-3,10(14),11(13)-三烯-12,6α-内酯(5)、artanomalide(6)、5-epi-seco-tanapartholide A(7)、seco-tanapartholide B(8)、iso-seco-tanapartholide(9)、3-epi-iso-seco-tanapartholide(10)。结论 10个化合物均为首次从柳蒿中分离得到,它们对HeLa细胞增殖均显示抑制活性,其中化合物1、4、7的活性最强,作用48、72 h后的半数抑制浓度(IC50)值均小于7.8 μg/mL。
[Key word]
[Abstract]
Objective To study the chemical constituents of Artemisia integrifolia. Methods The chemical constituents were separated and purified by column chromatographies and HPLC. Their structures were determined by spectroscopic analyses. Guaiane-type sesquiterpenoids were tested for their anticancer activity against uterine cancer (HeLa) by MTT method. Results Ten compounds were isolated from A. integrifolia with the structures identified as 3-methoxy-tanapartholide (1), dehydroleucodin (2), 4β-hydroxyguaia-2,10(14),11(13)-trien-12,6α-olide (3), 4α-hydroxyguaia-2,10(14),11(13)-trien-12,6α-olide (4), 2β-hydroxyguaia-3,10(14),11(13)-trien-12,6α-olide (5), eartanomalide (6), 5-epi-seco-tanapartholide A (7), seco-tanapartholide B (8), iso-seco-tanapartholide (9), and 3-epi-iso-seco-tanapartholide (10). Conclusion All compounds are isolated from this plant for the first time. The results showed that all sesquiterpenoids had potent inhibiting effects on HeLa cells proliferation. The inhibitory effects of compounds 1, 4, and 7 were the highest (IC50 < 7.8 μg/mL).
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[基金项目]
黑龙江省教育厅基本业务专项理工重点项目(135209101);齐齐哈尔大学研究生创新基金项目(YJSCX2017-026X)