目的 研究黔产细锥香茶菜Isodon coetsa的化学成分。方法 采用硅胶、凝胶柱色谱、制备型HPLC等方法进行分离纯化，通过理化性质、光谱数据结合参考文献鉴定化合物结构。结果 从细锥香茶菜95%甲醇提取物中分离得到了19个化合物，分别鉴定为β-谷甾醇（1）、豆甾醇（2）、β-胡萝卜苷（3）、亚麻酸甲酯（4）、油酸-1，3-甘油二酯（5）、熊果酸（6）、齐墩果酸（7）、3β-羟基-乌索-20-烯-28-酸（8）、白桦脂酸（9）、苦参黄素（10）、山楂酸（11）、2α羟基-熊果酸（12）、2α，3α-二羟基-12-烯-28-乌苏酸（13）、2α，3α，24-三羟基-12-烯-28-齐墩果酸（14）、2α，3α，24-三羟基-12-烯-28-熊果酸（15）、芒柄花素（16）、大黄素（17）、尿嘧啶（18）、蔗糖（19）。结论 化合物2、4、5、9、11、13～19均为首次从该植物中分离得到，化合物11、17、19为首次从香茶菜属植物中分离得到。

Objective To investigate chemical constituents from Isodon coetsa distributed in Guizhou. Methods Chemical constituents were isolated and purified by chromatography with silica gel, Sephadex LH-20, and semi-preparative HPLC, and their structures were identified by analysis of spectroscopic evidences and physicochemical properties as well as relevant references. Results A total of 19 compounds were isolated from plant material extracted with 95% aqueous methanol, which were elucidated as β-sitosterol (1), stigmasterol (2), β-daucosterol (3), methyl linolenat (4), 9,12-octadecadienoic acid-2-hydroxy-1,3-propanedinyl ester (5), ursolic acid (6), oleanoic acid (7), 3β-hydroxy-urs-20-en-28-oic acid (8), betulinic acid (9), kurarinone (10), maslinic acid (11), 2α-hydroxy ursolic acid (12), 2α,3α-dihydroxy-urs-12-en-28-oic acid (13), 2α,3α,24-trihydrxyolean-12-en-28-oic acid (14), 2α,3β,24-trihydroxyolean-12-en-28-oic acid (15), formononetin (16), emodin (17), uracil (18), and sucrose (19). Conclusion Compounds 2, 4, 5, 9, 11, 13-19 are isolated from this plant for first time, and compounds 11, 17, 19 are isolated from the genus of Isodon for the first time.

国家自然科学基金资助项目（81760772）；贵州省中管局项目（QZYY-2016-098）；GNYL（2017）008