[关键词]
[摘要]
目的 研究红葱Eleutherine americana的化学成分。方法 采用硅胶柱色谱、薄层色谱、Sephadex LH-20柱色谱、半制备高效液相色谱等方法进行分离纯化,通过理化性质及波谱数据鉴定化合物结构。结果 从红葱中分离出15个化合物,分别鉴定为大根香叶烯B(1)、phaffiaol(2)、4,8-dihydroxy-3-methoxy-l-methylonthra-9,10-quinone-2-carboxylic acid methyl ester(3)、3-heptadecyl-5-methoxyphenol(4)、亚油酸乙酯(5)、3,5-dimethoxybiphenyl-4'-ol(6)、karwinaphthol A(7)、5-hydroxylkarwinaphthol A(8)、3,4-dimethoxy-8-hydroxy-1-methyl-anthra-9,10-quinone-2-carboxylic acid methyl ester(9)、异红葱乙素(10)、红葱乙素(11)、异红葱甲素(12)、naphtho-γ-lactone (+)-9-hydroxyeleutherol(13)、洋川芎醌(14)、棕榈油酸(15)。结论 化合物2、4~7、13~15均为首次红葱属植物中分离得到,化合物3、8、13的舒张血管作用较强,舒张率分别达到了85.3%、81.8%、89.5%,基本与阳性药丹参酮ⅡA(86.3%)相当。
[Key word]
[Abstract]
Objective To study the chemical constituents of Eleutherine americana. Methods The compounds were isolated by various chromatographic techniques, including silica gel, TLC, sephadex LH-20, and semi-preparative HPLC, and their structures were identified by their physicochemical properties and 1H-NMR and 13C-NMR data. Results Fifteen compounds were isolated from E. americana, which were germacrenin B (1), phaffiaol (2), 4,8-dihydroxy-3-methoxy-l-methylonthra-9,10-quinone-2-carboxylic acid methyl ester (3), 3-heptadecyl-5-methoxyphenol (4), ethyl linoleate (5), 3,5-dimethoxybiphenyl-4'-ol (6), karwinaphthol A (7), 5-hydroxylkarwinaphthol A (8), 3,4-dimethoxy-8-hydroxy-1-methyl-anthra-9,10-quinone-2-carboxylic acid methyl ester (9), isoeleutherin (10), eleutherin (11), isoeleutherol (12), naphtho-γ-lactone (+)-9-hydroxyeleutherol (13), senkyunone (14), and palmitoleic acid (15). Conclusion Compounds 2, 4-7, and 13-15 were isolated from the genus for the first time. The compounds 3, 8, and 13 had strong vasodilator effects with diastolic rate of 85.3%, 81.8%, and 89.5%, respectively, which were basically equivalent to the positive drug of tanshinone ⅡA (86.3%).
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[基金项目]
海南省重点研发计划(ZDYF2017127);宁夏医科大学校级项目(XM2016093)