目的 研究金佛山雪胆Hemsleya pengxianensis var.jinfushanensis块根醋酸乙酯部位的化学成分。方法 利用硅胶柱、ODS反相柱、HPLC制备柱等多种色谱技术对金佛山雪胆块根醋酸乙酯部位的化学成分进行分离纯化，根据化合物的IR、HR-ESI-MS及NMR数据鉴定化合物结构。结果 从金佛山雪胆块根醋酸乙酯部位中分离得到3个化合物，分别鉴定为3β，26-二羟基-葫芦-5，24（E）-二烯-7，11-二酮-3-O-β-D-葡萄吡喃糖基-26-O-β-D-葡萄吡喃糖基（1→6）-β-D-葡萄吡喃糖苷（1）、3β，11α，26-三羟基-葫芦-5，24（E）-二烯-3-O-β-D-葡萄吡喃糖基-26-O-β-D-葡萄吡喃糖基（1→6）-α-L-阿拉伯糖苷（2）和3β，27-二羟基-葫芦-5，24（Z）-二烯-3-O-β-D-葡萄吡喃糖基-27-O-β-D-葡萄吡喃糖基（1→6）-β-D-葡萄吡喃糖苷（3）。结论 化合物1～3为新化合物，依次命名为金佛山皂苷Q、金佛山皂苷R、金佛山皂苷S。

Objective To study the chemical constituents of the tubers of Hemsleya pengxianensis var. jinfushanensis. Methods The compounds of the ethyl acetate fraction were isolated by silica gel, ODS reversed phase column chromatography, and preparative high-performance liquid chromatography (prep-HPLC). The structures of these compounds were identified by IR, HRESIMS, and NMR spectra. Results Three new compounds were obtained from the ethyl acetate fraction of the tubers of H. pengxianensis var. jinfushanensis and identified as 3β,26-dihydroxy-cucurbita-5,24(E)-diene-7,11-dione-3-O-β-D-glucopyranosyl-26-O-β-D-glucopyranosyl (1→6)-β-D-glucopyranoside (1), 3β,11α,26-trihydroxy-cucurbita-5,24(E)-diene-3-O-β-D-glucopyranosyl-26-O-β-D-glucopyranosyl (1→6)-α-L-arabinoside (2), and 3β,27-dihydroxy-cucurbita-5,24(Z)-diene-3-O-β-D-glucopyranosyl-27-O-β-D-glucopyranosyl (1→6)-β-D-glucopyranoside (3). Conclusion Compounds 1-3 are new compounds named hemslepenside Q, hemslepenside R, and hemslepenside S, respectively.

山东省自主创新及成果转化专项（2014ZZCX02105）；山东省医学科学院医药卫生科技创新工程项目