目的 采用一测多评法对临床常用药对人参-柴胡及其制剂中的人参皂苷Rg₁、人参皂苷Re、人参皂苷Rb₁、柴胡皂苷a和柴胡皂苷d进行测定。方法 以人参皂苷Rg₁为参照，建立该成分与其他4种皂苷成分间的相对校正因子，并以此计算其他4种皂苷成分的含量；同时采用外标法实测5种成分的含量进行对比。使用不同色谱柱确定相对保留时间，评价一测多评法在人参-柴胡药对中测定皂苷成分的可行性和准确性。结果 建立了5种皂苷成分的定量控制方法，方法学考察结果良好。采用一测多评法及外标法测定人参-柴胡药对、小柴胡汤、开心解郁方中5种皂苷成分，采用t检验分析，一测多评法与外标法结果无显著性差异。结论 一测多评法适用于常用药对人参-柴胡中皂苷成分的含量测定，可为复方中人参-柴胡药对有效成分的测定提供借鉴，以及为复方质量控制方法的建立提供参考。

Objective To study the effects of ginsenoside Rg₁, ginsenoside Re, ginsenoside Rb₁, saikosaponin a, and saikosaponin d in Ginseng Radix et Rhizoma-Bupleuri Radix (GRRBR) herb pair and its preparations by using quantitative analysis of multi-components by single-marker (QAMS). Methods On the basis of ginsenoside Rg₁, the relative correction factors between the ginsenoside Rg₁ and the other four saponins were established, and then the contents of the other four saponins were calculated. At the same time, the contents of the five components were determined by external standard method and compared with those evaluated by QAMS. The relative retention time was determined by different chromatographic columns. It could be considered that QAMS was feasible and accurate in the determination of saponins in GRRBR herb pair. Results A quantitative control method of five kinds of saponins was established, and the methodological results were good. The results showed that there was no significant difference in the contents of five kinds of saponins in GRRBR herb pair, Xiaochaihu Decoction, and Kaixin Jieyu Prescription between QAMS group and external standard method group. Conclusion QAMS is suitable for the determination of saponin in GRRBR herb pair, which can be used as a reference for the determination of its effective components and the establishment of compound quality control method.

北京市科委课题（Z161100001816013，Z171100001717012）