目的 建立超快速液相色谱-串联四级杆/线性离子阱质谱（QTRAP-UFLC-MS/MS）同时测定不同产地番荔枝子中氨基酸和核苷类成分含量的方法，分析比较不同产地样品中25种成分的差异，为番荔枝子资源的综合利用提供科学依据。方法 采用Waters XBridge Amide色谱柱（100 mm×2.1 mm，3.5μm）；以0.2%甲酸水（A）-0.2%甲酸乙腈（B）为流动相，梯度洗脱，体积流量0.6 mL/min；柱温30℃，正离子多反应监测（MRM）模式测定。采用主成分分析（PCA）和TOPSIS分析对不同产地番荔枝子进行综合性评价。结果 15种氨基酸和10种核苷类成分的浓度与峰面积呈良好的线性关系（r²>0.994 7）；方法精密度、重复性和稳定性的RSD值均小于3.87%；平均加样回收率在94.90%~98.78%，RSD在0.84%~3.01%。不同产地番荔枝子中氨基酸和核苷类成分存在一定的差异性，其中海南产的综合质量最高。结论 该方法简便、准确、可靠，可根据目标成分优选不同产地的番荔枝子，也为其质量评价提供了可靠的依据。

Objective To establish a QTRAP-UFLC-MS/MS method for the determination of 15 kinds of amino acids and 10 kinds of nucleosides in the seeds of Annona squamosa from different producing areas, toanalyze and investigate the difference of the 25 components in samples from five habitats, and provide scientific basis for its comprehensive utilization.Methods The analysis was carried out on a Waters XBridge Amide column (100 mm×2.1 mm, 3.5 μm) with elution by mobile phase of 0.2% formic acid in water (A)-0.2% formic acid in acetonitrile (B) at a flow rate of 0.6 mL/min. The column temperature was maintained at 30℃. The target compounds were analyzed by the positive ion multiple reaction monitoring (MRM) mode. The comprehensive evaluation of the seeds from A. squamosa in five habitats was carried out by PCA and TOPSIS analysis.Results All 15 kinds of amino acids and 10 kinds of nucleosides showed good linearity (r² > 0.994 7). The RSDs of the precision, repeatability, and stability tests were less than 3.87%. The average recovery rates were in the range from 94.90% to 98.78%, RSDs were in the range from 0.84% to 3.01%. There are differences in the composition and content of amino acids and nucleosides in the seeds of A. squamosa from different habitats, among which Hainan produced the highest comprehensive quality.Conclusion The method is simple, accurate, and reliable, which can provide the reliable basis for quality evaluation of the seeds from A. squamosa, and samples from different habitats can be optimized according to the target component.

国家自然科学基金资助项目（81274057，81573577）