目的 采用UPLC-Q-TOF/MS技术首次对北青龙衣化学成分的胃组织分布进行深入研究。方法 大鼠ig给予北青龙衣醇提物后，采集胃组织，经有机溶剂沉淀蛋白，通过Peakview 2.0/Masterview 1.0和MetabolitePilot 1.5数据处理软件，根据离子碎片精确质量数和同位素丰度比，数据库的二级质谱对比和裂解规律分析，对胃组织中移行成分进行推断和鉴定。结果 共鉴定出54个移行成分，其中41个原型成分，包括17个萘醌、9个二芳基庚烷、7个黄酮、5个三萜及3个酚酸类成分；13个代谢产物，包括4个萘醌、3个二芳基庚烷、1个黄酮及5个其他类成分。结论 基于UPLC-Q-TOF/MS技术，首次阐明北青龙衣化学成分在大鼠胃组织分布情况，该方法检测快速、准确，为北青龙衣抗胃癌药效学研究与深入开发提供科学的研究数据。

Objective To research the chemical constituents distribution from the exocarps of Jugland mandshurica in rats' stomach based on UPLC-Q-TOF/MS technology.Methods After ig administration with the exocarps of J. mandshurica, the tissue samples of stomach collected from each rat. Tissue samples were treated with organic solvent for protein precipitation. Peakview 2.0/masterview 1.0 and Metabolitepilot1.5 software were used to determine the constituents from the exocarps of J. mandshurica based on the acquired accurate mass, isotope abundance, and MS/MS fragment mass spectra.Results In total, 54 constituents were identified, including 41 prototypes and 13 metabolites. The prototypes consists of 17 naphthoquinone, 9 diarylheptanoids, 7 flavonoids, 5 triterpenes, and 3 phenolicacids. And metabolites consists of 4 naphthoquinones, 3 diarylheptanoids, 1 flavonoids, and 5 other main components.Conclusion The work was deeply researched the constituents from the exocarps of J. mandshurica in rats' stomach for the first time based on UPLC-Q-TOF/MS technique. It was demonstrated that this method could be successfully applied for rapid screening and deeply analysis of chemical constituents in vitro. This research provides scientific research data for exocarps of J. mandshurica against gastric cancer.

国家自然科学基金资助项目（81503348）；黑龙江省自然科学基金（H2017044）；国家中医药管理局中医药科学技术研究专项（2015-14）