[关键词]
[摘要]
目的 研究中药刺五加Acanthopanax senticosus的抗疲劳活性成分。方法 采用硅胶、Sephadex LH-20、MCI gel CHP-20P、ODS等多种色谱技术分离纯化,通过质谱及核磁共振等数据对化合物进行结构鉴定;并采用ABTS法研究其体外清除自由基活性。结果 从刺五加甲醇提取物中分离得到1个倍半萜类化合物7α(H)-桉烷-4α,5β,11,12-四醇-3-酮(1),该化合物自由基清除的半数清除浓度为(43.1±1.2)μg/mL。结论 化合物1为1个新的eudesmane型倍半萜,命名为刺五加醇A;也是首次从五加科植物中分离得到此类化合物。
[Key word]
[Abstract]
Objective To study the antifatigue bioactive constituents from Acanthopanax senticosus.Methods The compounds were isolated by means of various chromatographic techniques (Silica gel, Sephadex LH-20, MCI GEL CHP-20P, and HPLC), and its structures were elucidated by extensive spectroscopic analysis (IR, HR-MS, 1D-and 2D-NMR). The free radical scavenging activity in vitro was assessed by an ABTS assay.Results One new eudesmane sesquiterpenoid was isolated and identified as 7α(H)-eudesm-4α,5β,11,12-tetraol-3-one, and its scavenging activities towards ABTS free radical with half scavenging concentration of (43.1 ±1.2) μg/mL.Conclusion Compound 1 is a new eudesmane sesquiterpenoid named senticosol A. Meanwhile, this compound with eudesmane skeleton is also obtained from the Araliaceae for the first time.
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[基金项目]
国家自然科学基金青年基金资助项目(31600279)