[关键词]
[摘要]
近年来免疫分析技术在中医药领域广泛应用,尤其是在中药质量控制、中药安全性评价、中药(复方)机制研究等方面发挥了重要作用,其中中药小分子单克隆抗体的制备是这些应用的前提和关键。主要从完全抗原制备、动物免疫、杂交瘤细胞株建立及单克隆抗体生产等方面进行综述,特别对近20年已报道且具有代表性的中药小分子抗原抗体品种进行梳理,为中药小分子单克隆抗体的研究及应用提供参考。
[Key word]
[Abstract]
In recent years, immunoassay has been widely used in traditional Chinese medicine. It plays an important role in the quality control, the safety evaluation, and the mechanism research of Chinese medicine or compound medicine. Among them, the premise and key of the application is the preparation of monoclonal antibodies of small moleculars. This review summarized preparation of complete antigen, animal immunization, establishment of hybridoma cell lines, and production of monoclonal antibody. Especially, the varieties of representative antigen and antibody reported in recent 20 years were combed, which will provide the reference for the research and application of small molecular monoclonal antibodies of Chinese materia medica.
[中图分类号]
[基金项目]
天津市科技计划项目(17YFCZZC00350)