[关键词]
[摘要]
目的 制备15批川芎饮片标准汤剂,并对其进行质量研究。方法 按标准汤剂制备条件,制备15批川芎饮片标准汤剂,采用HPLC同时测定川芎饮片标准汤剂中阿魏酸、绿原酸和咖啡酸的含量;采用紫外光谱法和红外光谱法,建立川芎标饮片准汤剂指纹图谱;并利用相关系数法和双指标分析法,对不同批次川芎饮片标准汤剂的光谱图进行比对分析。结果 15批川芎饮片标准汤剂中阿魏酸、绿原酸和咖啡酸的质量分数分别为0.316 0%~0.565 6%、0.017 7%~0.050 4%、0.485 7%~0.749 5%,出膏率为18.82%~27.60%;与紫外和红外对照指纹图谱相比,各批次川芎饮片标准汤剂指纹图谱相似度分别大于0.990和0.911,双指标分析法结果显示各批次川芎饮片标准汤剂共有峰率均>51.72%。结论 建立的质量评价方法可用于川芎饮片标准汤剂的系统性评价,并为川芎配方颗粒等相关制剂的质量标准制定提供理论参考。
[Key word]
[Abstract]
Objective To prepare 15 batches of Chuanxiong Rhizoma standard decoctions and evaluate its quality.Methods According to preparation conditions of the TCM standard decoction, 15 batches of Chuanxiong Rhizoma standard decoction were prepared; The content of ferulic acid chlorogenic acid and caffeic acid in Chuanxiong Rhizoma standard decoction were determined by using HPLC; The fingerprint of Chuanxiong Rhizoma standard decoction was established by using UV-visible spectrophotometry and infrared spectroscopy; And diverse batches of Chuanxiong Rhizoma standard decoction fingerprint were comparative analyzed by using the correlation coefficient method and double-index analysis method.Results The results of 15 batches of samples revealed that the transfer rate of ferulic acid ranged from 0.485 7% to 0.749 5%, the transfer rate of chlorogenic acid ranged from 0.316 0% to 0.565 6%, the transfer rate of caffeic acid ranged from 0.017 7% to 0.050 4%, and extract rate was at the range of 18.82%-27.60%. Compared with the UV and IR standard fingerprint, the similarity of Chuanxiong Rhizoma standard decoction fingerprint was more than 0.990 and 0.911 respectively. The results of double-index analysis showed that the common peak rate of each lot of Chuanxiong Rhizoma standard decoction was more than 51.72%.Conclusion This quality evaluation method can be used for the systematic evaluation of Chuanxiong Rhizoma standard decoction. It also provides a theoretical reference preparation for the formulation of quality standard of for the formulation of quality standard for Chuanxiong Rhizoma preparations such as Chuanxiong Rhizoma dispensing granules.
[中图分类号]
[基金项目]
四川省科技厅2018年科技项目(2018TJPT0049,2018ZR0107);四川千人计划支持项目(2013332)