目的 采用新型绿色溶剂天然低共熔溶剂（nature deep eutectic solvents，NADESs）提取玉竹总黄酮，并对其提取工艺、提取效率、溶剂回收利用率进行考察。方法 采用响应面法优化玉竹总黄酮提取工艺；通过文献比较评价NADESs提取效率；并结合大孔树脂吸附法回收NADESs。结果 由醋酸钠和乳酸合成的NADESs （物质的量比为1：5）具有最高的提取率，经响应面法优化后，在水比例（水体积与溶液体积比）22%，提取温度51℃，提取时间21 min和液固比21 mL/g下，总黄酮提取率可达20.13 mg/g；而且，NADESs提取率显著高于文献报道的已有方法；回收实验表明，NAK-9对总黄酮的吸附效率（88.67%）显著高于D-101（77.33%）和AB-8（79.15%）。以甲醇为溶剂，其解吸附率达80.46%。回收后的NADESs重复使用的提取率达18.79 mg/g，回收利用率为94.56%。结论 NADESs作为提取介质具有高效、绿色环保及可重复利用等优点，可用于玉竹总黄酮类化合物的提取，同时为NADESs作为提取介质应用于其他中药有效组分的提取提供了新思路。

Objective To investigate the total flavonoids extraction process, solvent extraction rate, solvent recovery, and utilization using a new green solvent that nature deep eutectic solvents (NADESs) extraction of total flavonoids from Polygonati Odorati Rhizoma.Methods The extraction technology of total flavonoids was optimized by response surface methodology. The extraction efficiency of NADESs was evaluated by literature comparison and NADESs was recovered by macroporous resin adsorption.Results The NADESs (mole ratio 1:5) synthesized by sodium acetate and lactic acid was the best medium for extraction. After optimization by response surface methodology, the extraction rate of total flavonoids reached 20.13 mg/g at the best extraction condition of water 22%, extraction time 51℃, extraction time 21 min and liquid solid ratio 21 mL/g. The extraction rate of NADESs was significantly higher than the existing methods reported in the literature. The recovery experiments showed that the adsorption efficiency of NAK-9 on total flavonoids (88.67%) was significantly higher than that of D-101 (77.33%), and AB-8 (79.15%). Using methanol as solvent, the total flavonoids in Polygonati Odorati Rhizoma desorption rate was 80.46%. The recovered NADESs can be used for the extraction of total flavonoids in the next round, the extraction rate was 18.79 mg/g, and the recovery and utilization rate of solvent is 94.56%.Conclusion NADESs has the advantages of high efficiency, green environmental protection and can be reused as extraction medium, can be used for the extraction of total flavonoids from Polygonati Odorati Rhizoma. Meanwhile, the result provides a new idea that NADESs as an extraction medium for the extraction of other effective components of traditional Chinese medicine.

国家自然科学基金资助项目（81503041）；国家科技部公益性行业专项（201507002）；湖南省自然科学基金项目（2017JJ4045）；湖南省重点研发计划项目（2017SK2125）；2017年湖南省研究生科研创新项目（CX2017B440）