目的 研究膜翅目昆虫提取物Ento-Ⅱ涂膜剂的镇痛及活血化瘀作用，为相关药物的开发提供科学依据。方法 采用经典热板法和扭体法作为镇痛实验模型，观察Ento-Ⅱ涂膜剂对小鼠的镇痛作用；大鼠sc大剂量盐酸肾上腺素后，施以冰水浸泡制备急性血瘀模型，采用线栓法复制大鼠局灶性脑缺血再灌注损伤模型，测定血液流变学相关指标。结果 在镇痛实验中，与对照组比较，Ento-Ⅱ涂膜剂20、10、5 mg/kg能显著延长小鼠给药后30、60、90 min的痛阈值，明显减少小鼠15 min内的扭体次数，显著延长小鼠扭体的潜伏期（P<0.01）。在血瘀合并脑缺血实验中，与模型组比较，Ento-Ⅱ涂膜剂6.67、3.33、1.67 mg/kg组24 h时大鼠神经功能评分和全血黏度显著降低（P<0.01）；3.33 mg/kg组大鼠血浆黏度和红细胞聚集指数显著降低（P<0.05）；6.67、3.33 mg/kg组大鼠卡松黏度显著降低（P<0.01）。结论 Ento-Ⅱ涂膜剂具有明显的镇痛作用和活血化瘀作用。

Objective To study the analgesic, activating blood and resolving stasis effect of Ento-Ⅱ plastic.Methods Hot plate procedure and torsion body method were used as analgesic experimental model to observe the analgesic effect of Ento-Ⅱ plastic.The acute blood stasis rats experimental model was established through rat subcutaneous injection of high-dose adrenaline hydrochloride and soaked in ice water.Then, the blood stasis focal cerebral ischemia-reperfusion models were established by suture method on the rats.The hemorrheologic indicators were determined.Results Compared with control group, Ento-Ⅱ plastic could significantly prolong pain thresholds in mice at 30, 60, and 90 min after final administration (P < 0.01).Ento-Ⅱ plastics 20, 10, and 5 mg/kg dose groups could reduce aceticacid-induced mice writhing times and prolong the incubation period (P < 0.01).The experimental result of rats blood stasis focal cerebral ischemia-reperfusion at 24 h after reperfusion showed that Ento-Ⅱ plastics 6.67, 3.33, and 1.67 mg/kg dose groups could obviously reduce the neurological function score and whole blood viscosity (P < 0.01).3.33 mg/kg dose group could significantly reduce plasma viscosity, erythrocyte aggregation index, and casson viscosity (P < 0.05).The effect were similar to that of ligustrazine.6.67 and 3.33 mg/kg dose groups could significantly reduce casson viscosity (P < 0.01).Conclusion Ento-Ⅱ plastic has obvious analgesic effect and activating blood and resolving stasis effect.

国家自然科学基金资助项目：景颇族传统昆虫药物胡蜂蜂毒对大鼠MCAO/R后BBB的保护作用及其潜在机制探索（81703742）；云南省应用基础研究重点项目：胡蜂蜂毒抗脑缺血活性及相关物质基础比较研究（2017FA050）