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[摘要]
目的 研究山茶属植物茶Camellia sinensis的芽叶化学成分。方法 利用硅胶柱色谱法、Sephadex LH-20凝胶柱色谱法、中压柱色谱法及半制备高效液相色谱等方法分离纯化,通过理化常数和NMR、MS波谱等方法鉴定化合物结构。结果 从茶叶甲醇提取物中分离得到三萜及其苷类化合物9个,分别鉴定为21β-羟基坡模酸(1)、坡模酮酸(2)、坡模酸(3)、乌苏酸(4)、地榆皂苷Ⅱ(5)、3β-[(α-L-arabinopyranosyl) oxy]-urs-12,19(20)-dien-28-oic acid(6)、齐墩果酸-3-O-α-L-吡喃阿拉伯糖苷(7)、3β-[(α-L-arabinopyranosyl) oxy]-urs-12,18-dien-28-oic acid(8)、20-epi-urs-12,18-dien-28-oic acid 3β-O-α-L-arabinopyranoside(9)。结论 化合物1为新化合物,化合物2、3、5~9为首次从山茶属植物中分离得到。
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[Abstract]
Objective To investigate the chemical constituents from the leaf of Camellia sinensis.Methods The chemical constituents were isolated and purified by repeated silica gel column chromatography, Sephadex LH-20 gel column chromatography, ODS column chromatography, high pressure flash chromatography and semi-preparative HPLC, and their structures were elucidated on the basis of physico-chemical constants and spectral analysis.Results Nine compounds were separated from C. sinensis, among them, four compounds were identified as triterpenoids and named as 21β-hydroxyl pomolic acid (1), pomonic acid (2), pomolic acid (3), and ursolic acid (4);The other five compounds were idetified as triterpenoid saponins and named as 3-O-α-L-arabinopyranosyl pomolic acid (5), 3β-[(α-L-arabinopyranosyl) oxy]-urs-12, 19(20)-dien-28-oic acid (6), oleanolic acid-3-O-α-L-arabinopyranoside (7), 3β-[(α-L-arabinopyranosyl) oxy]-urs-12, 18-dien-28-oic acid (8), and 20-epi-urs-12, 18-dien-28-oic acid 3β-O-α-L-arabinopyranoside (9).Conclusion Compound 1 is a new compound, compounds 2, 3, 5—9 are obtained from this genus for the first time.
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