目的 克隆灰毡毛忍冬突变型品种AP1基因（Lm-XL-AP1），并对其进行生物信息学和时空表达分析。方法 通过RACE技术克隆Lm-XL-AP1基因全长，运用生物信息学的方法进行基因同源性和相似性比较分析，预测其编码蛋白，并对其进行各种理化性质分析。运用荧光定量PCR（qRT-PCR）检测该基因在灰毡毛忍冬突变型植株不同花期和不同器官的相对表达量。结果 获得AP1基因，开放阅读框（ORF）长729 bp，编码242个氨基酸，与甘菊中MADS-box基因家族AP1基因相似性达80%，且包含MADS和K-box的保守序列。AP1蛋白无跨膜区域，定位于细胞核中；在灰毡毛忍冬突变型植株第6花期相对表达量最高，同时茎、叶中也有表达。结论 成功从灰毡毛忍冬突变型植株总RNA中克隆到可能参与控制花器官表达的AP1基因。

Objective To clone the AP1 (Lm-XL-AP1) gene from a Special Variant Varieties of Lonicera macranthoides "Xianglei", and to analyze its bioinformatics and spatio-temporal expression. Methods Amplifing the full length of Lm-XL-AP1 gene by RACE technique, using bioinformatics method to analyze homology and similarity of the gene, predicting the coding protein and analyzing the various physical and chemical properties. The expression of the gene in different parts of Lonicera macranthoides Special Variant Varieties was detected by fluorescence quantitative PCR (qRT-PCR). Results The AP1 gene, containing a 729 bp ORF that encoding 242 amino acids, was cloned. And the similarity of the gene compared with the AP1 gene from the MADS-box gene family of Chrysanthemum lavandulifolium up to 80% (Containing a conserved sequence of MADS and K-box). Without transmembrane domain, AP1 was located in cell nucleus. It is expressed in various organs of Lonicera macranthoides Special Variant Varieties. Conclusion For the first time, the AP1 gene which may be involved in the control of the expression of floral organ was cloned from the total RNA of Lonicera macranthoides Special Variant Varieties.

国家自然科学基金资助项目（81203007，81673546）；湖南省自然科学基金资助项目（2017JJ3237）；湖南省教育厅青年基金项目（16B193）；湖南省中药学重点学科基金项目（zy201505）；湖南省"中药学"重点学科建设项目资助（湘教通[2011]76号）；国家中医药管理局"药用植物学"重点学科资助（国中医药发[2009]30号）；湖南省教育厅（14K071）；湖南中医药大学研究生省级创新课题