[关键词]
[摘要]
目的 研究红景天苷在大鼠体内的代谢物谱,分析其代谢途径。方法 大鼠ig给予红景天苷200 mg/kg后,收集胆汁、尿液、粪便和血浆,应用高分辨质谱技术鉴定可能的代谢产物,分析红景天苷在大鼠体内的代谢途径。结果 共检测到除原型药物外的20个代谢产物,提示红景天苷在大鼠体内的主要代谢途径为葡萄糖化、乙酰化、硫酸化和甲基化。结论 红景天苷在大鼠体内经历了广泛的I相和Ⅱ相代谢,代谢物主要通过尿液排出。应用峰面积归一化法计算主要代谢产物相对生成量,提示红景天苷在大鼠尿、粪、胆汁中的主要代谢途径为Ⅱ相代谢,血浆中红景天苷原型药物为体循环系统中主要暴露物质。
[Key word]
[Abstract]
Objective To analyze the metabolic pathways of salidroside when gavaged to rats, and to identify its in vivo metabolites. Methods A UPLC-Q-TOF-MS method was established and applied to identify the metabolites of salidroside in bile, urine, feces, and plasma samples after ig administration of salidroside 200 mg/kg to rats. Results A total of 20 metabolites were characterized in rats. The dominant metabolic pathways of salidroside include glucuronidation, acetylation, sulfation, and methylation. Conclusion Salidroside undergoes extensive phases I and Ⅱ metabolism in rats. The main excretion path is the phase Ⅱ conjugated metabolites via urine, feces, and bile. The parent drug of salidroside is the main exposure material in plasma.
[中图分类号]
[基金项目]
国家自然科学基金青年科学基金项目(81503154)