目的 采用UPLC-Q-Exactive四极杆-静电场轨道阱高分辨质谱仪在正、负离子模式下分离鉴定川党参Codonopsis tangshen药材的化学成分。方法 Acquity UPLC BEH C₁₈色谱柱（100 mm×2.1 mm，1.7 μm）分离，以乙腈（A）-0.1%甲酸水溶液（B）为流动相进行梯度洗脱，质谱采用正、负离子监测模式、全扫描及自动触发二级质谱扫描功能，对川党参药材的化学成分进行快速分离和鉴定。结果 根据高分辨质谱数据从川党参50%甲醇提取物中共鉴定出36个化合物，包括生物碱类7个、苯丙素类7个、多炔类3个、己醇苷与己烯醇苷6个、有机酸类8个、氨基酸类3个、核苷类1个、倍半萜类1个；其中9个成分经与对照品比对而准确鉴定，19个成分在该植物中首次发现，1个成分为潜在的新化合物。结论 该方法可快速分离鉴定川党参的化学成分，为其质量控制和药效物质基础研究提供理论依据。

Objective The chemical constituents from the roots of Codonopsis tangshen were isolated and identified using ultra-high performance liquid chromatography coupled with Q-Exactive quadrupole-orbitrap MS spectrometry in positive and negative ion modes. Methods The 50% methanol extract from the roots of C. tangshen was isolated on an Acquity UPLC BEH C₁₈ column (100 mm×2.1 mm, 1.7 μm). The mobile phase was acetonitrile and water containing 0.1% formic acid by gradient elution. The constituents in the roots of C. tangshen were rapidly isolated and identified by HRMS in the positive and negative ion mode using both full scan and two stage threshold-triggered mass modes. Results A total of 36 compounds from the roots of C. tangshen were identified, including 7 alkaloids, 7 phenylpropanoids, 3 polyacetylenes, 6 hexanol glycosides and hexylene glycosides, 8 organic acids, 3 amino acids, 1 nucleoside, and 1 sesquiterpenoid. Among them, nine compounds were unambiguously identified comparing with the reference standards. Nineteen chemical components were reported in this plant for the first time. One of them is potential new compound.Conclusion Chemical components in the roots of C. tangshen were isolated and identified rapidly with UPLC-Q-Exactive quadrupole-orbitrap MS technology to provide the theory basis for the study on the quality control and pharmacodynamic material.

国家"十二五"重大新药创制专项（2014ZX09304307-002）；药品医疗器械审评审批制度改革子课题（中药）（ZG2016-2-06）