目的 采用HPLC法对10批不同厂家的千年健药材进行指纹图谱研究，并采用相似度计算的方法，评价千年健药材的质量。方法 采用HPLC法测定不同厂家的10批千年健药材。Phenomenex Gemini 110A C₁₈色谱柱（250 mm×4.6 mm，5μm），乙腈（A）-0.01%磷酸水溶液（B）为流动相进行梯度洗脱，柱温30℃，体积流量1.0 mL/min，检测波长290 nm。利用"中药色谱指纹图谱相似度评价系统2004A版"对指纹图谱进行相似度评价。结果 建立了千年健药材HPLC指纹图谱，确定了11个共有峰，指认了1个峰，除了S4和S10两批，指纹图谱的相似度达到0.887以上；所建立的千年健药材指纹图谱测定方法精密度、稳定性和重复性良好。结论 首次建立了千年健药材的HPLC特征指纹图谱，不仅为千年健药材的鉴别、质量控制等提供了新的科学依据，还对千年健药材的质量评价具有重要的意义。

Objective To establish an HPLC method for the fingerprint analysis of Homalomena occulta and evaluate the quality though similarity calculation. Methods Waters e2695 was applied to analyze ten batches of H. occulta. Phenomenex Gemini 110A C₁₈ column was performed (250 mm×4.6 mm, 5 μm) with acetonitrile and 0.01% phosphoric acid in agradient mode at a flow rate of 1.0 mL/min at 290 nm and the separation was performed at 30℃. The similarity was analyzed with Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica 2004A. Results The HPLC characteristic fingerprint of H. occulta with 11 commons peaks, and one of them were identified by comparing with reference subslances. The values of similarity was higher than 0.887. The HPLC method showed better results of stability, precision, and repeatability. Conclusion It is the first time to establish the HPLC fingerprint of H. occulta. Those described can not only provides an identification method for fingerprint, but also provides the foundation of quality control.