[关键词]
[摘要]
目的 建立远志药材UPLC指纹图谱及多指标性成分测定的方法,用于不同规格等级远志药材的质量评价。方法 采用UPLC法,建立不同规格等级远志药材的指纹图谱,并测定远志药材中sibiricose A5、sibiricose A6、lancerin、sibiricoxanthone B、glomeratose A、7-O-methyl mangiferin、polygalaxanthone Ⅲ、3,6'-disinapoyl sucrose、tenuifoliside A、tenuifoliside C 10种指标性成分的含量,同时建立综合质量评价函数。结果 建立的指纹图谱及含量测定方法符合方法学要求,指纹图谱共标定37个共有峰,指认出其中22个峰;不同规格等级远志药材样品间的相似度在0.927~0.971;10种指标性成分含量均有不同程度的差异,综合质量评价函数表明大筒远志药材样品的整体质量优于中筒远志药材样品,野生远志药材样品的整体质量优于栽培品。结论 建立的指纹图谱结合多指标成分测定的方法可用于远志药材样品的综合质量评价。
[Key word]
[Abstract]
Objective To establish a method of fingerprint analysis and determination of index compounds on commercial Polygala tenuifolia by ultra performance liquid chromatography, and to evaluate the quality of P. tenuifolia with different commodity specification grades. Methods UPLC was used to establish different commodity specification grade of P. tenuifolia and determine the contents of ten indicative compounds including sibiricose A5, sibiricose A6, lancerin, sibiricoxanthone B, glomeratose A, 7-O-methyl mangiferin, polygalaxanthone Ⅲ, 3,6'-disinapoyl sucrose, tenuifoliside A, and tenuifoliside C, and establish comprehensive quality evaluation function at the same time. Results The methods for fingerprint analysis and compound determination were in agreement with methodological requirements. A total of 37 peaks were selected as the common peaks, of which 22 peaks were identified, and the similarities were between 0.927 and 0.971. There are differences at different degrees in the contents of ten indicative compounds. The comprehensive quality evaluation function showed that the overall quality of Datong was better than that of Zhongtong; The overall quality of wild P. tenuifolia was better than that of cultivated. Conclusion The validated UPLC fingerprint analysis and compound determination methods were successfully used in the quality control of P. tenuifolia.
[中图分类号]
[基金项目]
国家科技支撑计划(2011BAI07B05);山西省重点研发计划重点项目(201603D3111003);山西省应用基础研究项目(201601D011064);国家中药标准化项目(ZYBZH-Y-JIN-34);中央本级重大增减支项目"名贵中药资源可持续利用能力建设"(2060302);山西省科技攻关项目(20140313010-1);地产中药功效物质研究与利用山西省重点实验室(201605D111004);山西省科技创新重点团队(201605D131045-18)