[关键词]
[摘要]
目的 采用叶绿体基因psb A-trnH间隔区序列探讨不同产地何首乌的分子鉴定方法。方法 分别提取7省区15个居群116份何首乌样品的总DNA,经PCR扩增psb A-trnH间隔区序列,纯化后测序,采用MEGA 6.06软件对序列进行分析。结果 何首乌各居群间的遗传距离为0.001~0.187,最大似然法系统树中何首乌15个居群样品聚为2支。结论 何首乌的遗传变异较显著,psbA-trnH序列可作为道地种源德庆种源与其他种源分子鉴定的依据。
[Key word]
[Abstract]
Objective The molecular identification method of Polygonum multiflorum from different producing areas was explored by using the sequencing of intergenic region of chloroplast genes psbA-trnH. Methods A total of 116 samples of P. multiflorum were collected from 15 populations in seven provinces and autonomous regions. The total DNA was extracted and the sequences of psbA-trnH were amplified by PCR. The purified PCR products were sequenced and analyzed by MEGA 6.06 software. Results The genetic distances among the populations of P. multiflorum are 0.001-0.187. In the maximum likelihood phylogenetic tree, 15 populations of P. multiflorum were clustered into two blanches. Conclusion The genetic variation of P. multiflorum is significant and the psbA-trnH sequences of P. multiflorum can be used as germplasm source for molecular identification between Deqing regarded as geo-authentic habitat and other producing areas.
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[基金项目]
广东省中医药局科研项目(20163010,20182076);大学生创新创业训练计划项目(201710573016);广东省科技厅项目(2017A020213023)