目的 建立酸枣仁黄酮部位不同配伍的HPLC指纹图谱，研究其与解郁安神功效的谱效关系。方法 采用Spearman分析、灰色关联度分析、多元回归分析方法将HPLC指纹图谱中各组分特征峰的峰面积与小鼠行为学指标数据相关联。结果 酸枣仁黄酮部位HPLC指纹图谱中与药效有较强相关性的成分有5个，分别为维采宁-Ⅱ、牡荆素葡萄糖苷、斯皮诺素、6'"-阿魏酰斯皮诺素、6'"-对羟基苯甲酰斯皮诺素。结论 通过谱-效相关性分析，推测出酸枣仁黄酮部位发挥抗抑郁、镇静催眠作用的有效成分，为酸枣仁有效成分筛选及质量控制方法的建立提供了依据。

Objective To establish the HPLC fingerprints of different compatibility of flavonoids in Ziziphi Spinosae Semen (ZSS) so as to establish spectrum-efficient correlation between HPLC fingerprints and the effects of depression-resolving and spirit-tranquilizing. Methods Spearman analysis, grey relational analysis, and multiple regression analysis were used to correlate the peak area of the characteristic peaks of each component in the HPLC fingerprint with the behavior indexes of depressive and insomnia mice. Results There were five chemical compositions strongly related with efficacy of flavonoids in ZSS, including vicenin-Ⅱ, glucosylvitexin, spinosin, 6'"-feruloylspinosin, and 6'"-p-hydroxybenzoylspinosin. Conclusion The effective components of flavonoids in ZSS with depression-resolving and spirit-tranquilizing effects can be inferred by spectrum-efficient correlation analysis, which can provide the basis for the screening and quality control of effective components of ZSS.

国家自然科学基金资助项目（81173530）；天津市自然科学基金重点项目（17JCZDJC33200）