[关键词]
[摘要]
目的 定性分析大鼠口服芩百清肺浓缩丸后肺组织中的化学成分。方法 ig给予大鼠芩百清肺浓缩丸,采用UPLC-Q-TOF-MS技术,对比供试品、空白组织及给药后肺组织中的化学成分,结合Peakview和Metabolitepilot软件,根据所提供的保留时间、精确相对分子质量及二级质谱裂解碎片鉴定和表征其肺组织中化学成分。结果 在给药后的大鼠肺组织中分析表征了25个化学成分,其中17个原型成分,8个代谢产物。结论 借助UPLC-Q-TOF-MS方法鉴定了大鼠口服芩百清肺浓缩丸后肺组织中的化学成分,有助于阐明该方发挥治疗支原体肺炎作用的潜在效应物质基础。
[Key word]
[Abstract]
Objective To qualitative analze the chemical components in rat lung tissues after oral administration Qinbai Qingfei Concentrated Pellets (QQCP). Methods The rat lung tissues were collected and analyzed by UPLC-Q-TOF-MS after ig administration of QQCP. The chemical components in control and dose groups were identified and speculated by Peakview and Metabolite Pilot data processing software using retention time, exact relative molecular mass, and cleavage fragments of MS/MS as indexes. Results A total of 25 compounds were identified, including 17 prototypes and eight metabolites. Conclusion The chemical constituents in the rat lung tissues were identified after oral administration of QQCP based on UPLC-Q-TOF-MS, which will contribute to elucidate the effects of potential pharmacodynamic material basis of QQCP on mycoplasma pneumoniae.
[中图分类号]
[基金项目]
国家自然科学基金资助项目(81374045);黑龙江省应用技术研究与开发计划(PB15F005);哈尔滨市青年科技创新人才基金项目(2016RAQYJ198)