[关键词]
[摘要]
目的 研究羊踯躅Rhododendron molle果实中的化学成分。方法 利用硅胶柱色谱、ODS柱色谱、Sephadex LH-20凝胶柱色谱、半制备高效液相、重结晶等多种方法进行分离和纯化,根据理化性质和波谱技术进行结构鉴定。结果 从羊踯躅果实中分离得到15个化合物,鉴定其结构分别为杨梅素(1)、双氢杨梅素(2)、没食子儿茶素(3)、槲皮素-3'-O-葡萄糖苷(4)、儿茶素(5)、表儿茶素(6)、二氢槲皮素3'-O-葡萄糖苷(7)、原花青素A-2(8)、去氢双儿茶素A(9)、槲皮素-3-O-α-阿拉伯糖苷(10)、根皮苷(11)、南烛木树脂酚3-O-吡喃鼠李糖苷(12)、2,6-二甲氧-4-羟基苯酚-1-O-葡萄糖苷(13)、2,4,6-三羟基苯乙酮-2-O-吡喃葡萄糖苷(14)、5'-β-吡喃葡萄糖-O-茉莉酸(15)。结论 化合物1~4、7~9、13~15首次从羊踯躅植物中分离得到,其中化合物1~11为黄酮类化合物。
[Key word]
[Abstract]
Objective To investigate the chemical constituents from the fruits of Rhododendron molle. Methods Compounds were isolated and purified by a series of methods including silica gel, ODS column, Sephadex LH-20, semi-preparative HPLC, recrystallization, and so on. Their structures were identified by analysis of physico-chemical propertiesand spectroscopic technique. Results Fifteen known compounds were isolated and their structures were elucidated as myricetin (1), dihydromyricetin (2), gallocatechin (3), quercetin-3'-O-glycoside (4), catechin (5), epicatechin (6), taxifolin 3'-O-glucopyranoside (7), proanthocyanidin A-2 (8), dehydroicatechin A (9), quercetin-3-O-α-arabinoside (10), phlorizin (11), lyoniresinol 3-O-rhamnopyranoside (12), 2,6-dimethoxy-4-hydroxyphenol-1-O-glucopyranoside (13), 2,4,6-trihydroxy acetophenone-2-O-glucopyranoside (14), and 5'-β-glucopyranosyloxy-jasmonic acid (15). Conclusion Compounds 1-4, 7-9, and 13-15 are obtained from R. molle for the first time. Compounds 5, 6, and 10-12 are obtained from the fruits of this plant for the first time, of which compounds 1-11 are flavonoids.
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[基金项目]
上海市卫生和计划生育委员会项目(ZY3-CCCX-3-5001);上海市科学技术委员会科研计划项目(14401901400,15DZ2292000);上海中医药大学预算内项目(2016YSN24)