目的 建立同时测定陕西产不同生长年限野生和栽培黄芩中野黄芩苷、黄芩苷、野黄芩素、汉黄芩苷、黄芩素、汉黄芩素、芹菜素、白杨素和千层纸素A 9种化学成分的HPLC方法。方法 黄芩药材粉末经乙醇（每次30 min）超声提取2次，合并提取液。色谱条件采用Ultrasil C₁₈色谱柱（250 mm×4.6μm，5 μm），乙腈-0.1%甲酸水溶液作为流动相，梯度洗脱；体积流量1 mL/min，检测波长为274 nm；柱温30℃。结果 所测9种主要有效成分在测定质量浓度范围内线性关系良好，r均大于0.999 5；精密度、重复性和稳定性良好；平均回收率为95.94%～99.88%，RSD为0.68%～1.90%。结论 建立的HPLC方法可用于同时测定黄芩中9种主要化学成分，该方法准确、简便、灵敏度高、专属性好，可为黄芩质量评价提供一定的参考。

Objective To establish an HPLC method for simultaneous determination of nine main chemical constituents in Scutellaria baicalensis (scutellarin, baicalin, scutellarin, wogonoside, apigenin, baicalein, wogonin, aspen, and melaleuca (A). Methods 0.5 g powders of S. baicalensis was extracted two times by ethanol (each for 30 min), and the extracts were merged into 50 mL. Separation was carried out on Ultrasil C₁₈ chromatography column (250 mm×4.6 μm, 5 μm), by using acetonitrile-0.1% formic acid water as the flow phase for gradient elution; with volume flow at 1 mL/min, detection wavelength at 274 nm and column temperature at 30℃. Results The linear relation of nine main active components measured in the range of mass concentration was good with perfect precision, repeatability and stability, the r value of which was all more than 0.999 5. The average recovery rate was 95.94%—99.88%, RSD was 0.68%—1.90%. Conclusion The HPLC method established for the simultaneous determination of nine main chemical compositions in S. baicalensis is accurate, simple, sensitive and specific, which can provide references for the quality evaluation of S. baicalensis.

陕西省科技厅项目：中药黄芩种质选育、种苗繁育与种植关键技术研究（2016KTTSSF01-01-01）