目的 研究连花清瘟胶囊化学成分。方法 采用硅胶、凝胶柱色谱、液相色谱等多种色谱方法对其进行分离和纯化，根据理化性质及波谱数据进行结构鉴定。结果 从连花清瘟胶囊孔树脂70%乙醇部位分离得到20个化合物，分别鉴定为芦荟大黄素（1）、连翘脂素（2）、cepharanone B（3）、松脂醇（4）、表松脂醇（5）、松脂素单甲醚（6）、胡椒内酰胺A（7）、反式对羟基肉桂酸乙酯（8）、刺芒柄花素（9）、异甘草素（10）、柚皮素（11）、山柰酚（12）、ω-羟基大黄素（13）、大黄酸（14）、大黄素甲醚-8-O-β-D-吡喃葡萄糖苷（15）、4，5-dioxodehydroasimilobine（16）、kaempferol-3-O-α-L-ramnopyranosyl-4″-O-E-（4-hydroxy）-cinnamoyl（17）、大黄酚-1-O-β-D-吡喃葡萄糖苷（18）、大黄酚-8-O-β-D-吡喃葡萄糖苷（19）、大黄素-8-O-β-D-吡喃葡萄糖苷（20）。结论 化合物2、3、5～17、19、20为首次从该复方中分离得到，为阐明连花清瘟胶囊药效物质奠定了基础。

Objective To study the chemical constituents of Lianhua Qingwen Capsule (LQC). Methods The compounds were isolated and purified by column chromatography over silica gel and sephadex LH-20, and preparative RP-HPLC. Their structures were elucidated by physicochemical properties and spectral analyses. Results Twenty compounds were isolated and identified as aloe-emodin (1), phillygenol (2), cepharanone B (3), pinoresinol (4), epipinoresinol (5), pinoresinol monomethyl ether (6), piperolactam A (7), (E)-ethyl 3-(4-hydroxyphenyl) acrylate (8), formononetin (9), isoliquiritigenin (10), naringenin (11), kaempferol (12), citreorosein (13), rhein (14), physcion-8-O-β-D-glucopyranoside (15), 4,5-dioxodehydroasimilobine (16), kaempferol-3-O-α-L-ramnopyranosyl-4″-O-E-(4-hydroxy)-cinnamoyl (17), chrysophanol-1-O-β-D-glucopyranoside (18), chrysophanol-8-O-β-D-glucopyranoside (19), and emodin-8-O-β-D-glucopyranoside (20). Conclusion Compounds 2, 3, 5—17, 19, and 20 are isolated from LQC for the first time. This study establishes the foundation for explaining the efficient substance of LQC.

国家自然科学基金青年基金项目（81503231）；国家科技重大专项（2017ZX09307002）；北京市科技计划（Z171100004517014）