[关键词]
[摘要]
目的 对东北茶藨子Ribes mandshuricum进行化学成分研究。方法 采用硅胶、ODS、Sephadex LH 20、MCI、半制备HPLC进行成分分离,并根据理化性质和波谱数据鉴定化合物的结构。结果 从东北茶藨子95%乙醇提取物的各萃取部位中分离得到27个化合物,分别鉴定为山柰酚(1)、山柰酚-7-O-β-D-葡萄糖苷(2)、山柰酚-3-O-α-L-阿拉伯糖苷(3)、山柰酚-3-O-α-L-阿拉伯糖基-7-O-α-L-鼠李糖苷(4)、山柰酚-7-O-α-L-鼠李糖苷(5)、山柰酚-3,7-二-O-α-L-二鼠李糖苷(6)、山柰酚-3-O-β-D-半乳糖基-7-O-α-L-鼠李糖苷(7)、山柰酚-3-O-β-D-葡萄糖基-7-O-α-L-鼠李糖苷(8)、槲皮素(9)、槲皮素-7-O-α-L-鼠李糖苷(10)、槲皮素-3-O-β-D-葡萄糖基-7-O-α-L-鼠李糖苷(11)、杨梅素-3,7-二-O-α-L-二鼠李糖苷(12)、槲皮素-3-O-α-L-鼠李糖基-7-O-β-D-半乳糖苷(13)、对羟基苯甲酸(14)、原儿茶酸(15)、香草酸(16)、pleoside(17)、丁香酸葡萄糖苷(18)、juniperoside(19)、4-羟基-3-甲氧基苯甲酸乙酯(20)、菜豆酸(21)、脱落酸(22)、jasminoside E(23)、conocarpan(24)、豆甾-4-烯-3,6-二酮(25)、豆甾-4-烯-3-酮(26)、24-ethylcholesta-4,24(28)Z-lien-3-one(27)。结论 化合物20可能为人工产物,其余化合物物均为首次从该植物中分离得到。
[Key word]
[Abstract]
Objective To study the chemical constituents of 95% ethanol extract of Ribes mandshuricum. Methods A total of 27 compounds were isolated from R. mandshuricum using a combination of solvent extraction, silica gel, ODS, Sephadex LH-20, MCI column chromatography, recrystallization, and semi-preparative HPLC. Their chemical structures were elucidated by physicochemical properties and spectroscopic methods. Results These compounds were determined as kaempferol (1), kaempferol-7-O-β-D-glucopyranoside (2), kampferol-3-O-α-L-arabinopyranoside (3), kaempferol-3-O-α-L-arabinofuranosyl-7-O-α-L-rhamnopyranoside (4), kaempferol-7-O-α-L-rhamnopyranoside (5), kaempferol-3,7-di-O-α-L-rhamnopyranoside (6), kaempferol-3-O-β-D-galactopyranosyl-7-O-α-L-rhamnopyranoside (7), kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnoside (8), quercetin (9), quercetin-7-O-α-L-rhamnopyranoside (10), quercetin-3-O-β-D-glucopyranosyl-7-O-α-L-rhamnopyranoside (11), myricetin-3,7-di-O-α-L-rhamno-pyranoside (12), quercetin-7-O-β-D-galactopyranosyl-3-O-α-L-rhamnopyranoside (13), p-hydroxybenzoic acid (14), protocatechuic acid (15), vanillic acid (16), pleoside (17), glucosyringic acid (18), juniperoside (19), ethyl 4-hydroxy-3-methoxybenzoate (20), phaseic acid (21), abscisic acid (22), jasminoside E (23), conocarpan (24), (24R)-24-ethylcholest-4-en-3,6-dione (25), (24R)-24-ethylcholest-4-en-3-dione (26), and 24-ethylcholesta-4,24(28)Z-lien-3-one (27). Conclusion Compound 20 might be an artifact presumably formed during the extraction process, and the others are isolated from the title plant for the first time.
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[基金项目]
国家自然科学基金面上基金资助项目(21777192,81473539);中国博士后科学基金资助项目(2015M571844);国家自然科学基金青年基金资助项目(81603389);江苏省自然基金资助项目(BK20141357,BK20160764,BK20160754)