目的 对拟茎点霉属真菌福士拟茎点霉Phomopsis fukushii发酵产物的化学成分进行研究。方法 运用硅胶、凝胶及RP-HPLC等多种色谱技术进行分离纯化得到化合物，并根据理化性质和波谱数据鉴定化合物的结构。结果 从福士拟茎点霉发酵产物中分离到4个异戊基二苯醚类化合物，分别鉴定为1-[4-（3-羟基-5-甲基苯氧基）-2-甲氧基-6-甲基苯]-3-甲基丁基-3-烯-2-酮（1）、diorcinol C（2）、diorcinol D（3）和diorcinol E（4）。对化合物1进行抗菌活性筛选，发现其对耐甲氧西林金黄色葡萄球菌（MRSA）菌株的MIC₉₀值为（54±4）μg/mL。结论 化合物1为新化合物，命名为拟茎点二苯醚A；化合物2～4为拟茎点属真菌中首次发现的异戊基二苯醚类化合物，化合物1具有突出的抗菌活性，接近阳性对照左氧氟沙星的MIC₉₀值。

Objective Try to find the new biological compounds, the research on the chemical constituents in the fermentation products of an endophytic fungus Phomopsis fukushii had been carried out. Methods The chemical constituents in this fermentation products were isolated by silica gel, Sephadex LH-20 column chromatographies and RP-HPLC methods. Their structures were elucidated by using various spectroscopic techniques. Results Four pentylated diphenyl ethers (1—4) were isolated from this fermentation products, and the new compound (1) was evaluated for its anti-methicillin-resistant Staphylococcus aureus (anti-MRSA) activity. Compounds 2—4 were identified as diorcinol C, diorcinol D, and diorcinol E. Conclusion Compounds 2—4 are isolated from the fermentation products of endophytic fungus Phomopsis fukushii for the first time. Compound 1 is a new compound named phomodiphenyl ether A and given the system name of 1-[4-(3-hydroxy-5-methylphenoxy)-2-methoxy-6-methylphenyl]-3-methylbut-3-en-2-one. Compound 1 also shows strong anti-MRSA activity with MIC₉₀ value of (54 ±4) μg/mL. This valve is close to that of positive control, levofloxacin with MIC₉₀ value of[ ≥ (56 ±6) μg/mL].

国家自然科学基金资助项目（21462051）；中国烟草总公司科技重点项目（110201502006）；云南中烟科技项目（2015539200340277）