目的 探究雌、雄大鼠肠道菌群对三七总皂苷中3种皂苷（三七皂苷R₁、人参皂苷Rg₁和人参皂苷Rb₁）的体外降解作用。方法 将三七总皂苷分别与雌、雄大鼠肠道菌群孵育液在厌氧条件下共同培养24 h，测定不同时间点孵育液中3种皂苷的量。结果 雌、雄大鼠肠道菌群对人参皂苷Rb₁均有降解作用，雄鼠的降解较雌鼠稍快。雌、雄鼠肠道菌群对三七皂苷R₁和人参皂苷Rg₁均无明显降解作用。结论 在离体条件下，三七总皂苷中人参皂苷Rb₁会被肠道菌群降解，而三七皂苷R₁和人参皂苷Rg₁则较为稳定。

Objective To investigate the degradation of three kinds of saponins (notoginseng saponin R₁, ginsenoside Rg₁, and ginsenoside Rb₁) from total saponins of Panax notoginseng (tPNS) by intestinal flora in male and female rats in vitro. Methods tPNS were incubated for 24 h with male and female rats intestinal flora separately in vitro under anaerobic environment. The content of three kinds of saponins was measured at different treatment time. Results The results showed that the intestinal flora both in male and female rats all had the degradation action against ginsenosides Rb₁, in which the degradation of male rats was slightly faster than that of female rats. But the intestinal flora both in male and female rats had no obvious degradation to notoginseng saponin R₁ and ginsenoside Rg₁. Conclusion Under the condition of in vitro, tPNS can be degraded by rats intestinal flora, of which Rb₁ was degraded most, while notoginseng saponin R₁ and ginsenoside Rg₁ were more stable.

国家自然科学基金面上项目（81673830）