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[摘要]
目的 通过建立超高效液相色谱-质谱联用(UPLC-Q-TOF-MS)分析方法,对加参片提取物化学成分进行定性分析,确定加参片提取物的主要化学成分。方法 采用Waters ACQUITY UPLC BEH C18色谱柱(100 mm×2.1 mm,1.7 μm),以0.1%甲酸水(A)-甲醇(B)为流动相梯度洗脱;体积流量0.3 mL/min,采用电喷雾离子化源(ESI),MSE扫描正负离子模式检测。结果 通过与对照品比对、数据库匹配、质谱数据分析及参考相关文献,从加参片提取物中共鉴定出68个化学成分,并发现了4个未知化合物,其中包括13个酚酸类成分、6个丹参酮类成分、11个黄酮及其苷类成分、4个强心苷类成分、15个三萜皂苷类成分、7个C21甾体类成分、12个其他类成分及4个未知成分。结论 对加参片提取物化学成分进行了较为全面的分析,为加参片的物质基础及质量控制研究打下了良好的基础。
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[Abstract]
Objective To establish a method for the major content identification of Jiashen Tablet extract by using UPLC-Q-TOF-MS. Methods The chromatographic separation was executed on a Waters ACQUITY UPLC BEH C18 column (2.1 mm×100 mm, 1.7 μm) with methanol-0.1% formic acid in water by gradient elution. Electrospray ionization (ESI) source was applied and operated in both positive and negative ion mode. Results A total of 72 compounds were found and 68 compounds among them were identified by the methods of comparing reference substance, matching Database and referencing related literature, including 13 phenolic acids, 6 tanshinones, 11 flavonoids and their glycosides, 4 cardiac glycosides, 15 triterpenoid saponins, 7 C21 steroids, and 13 other components. Conclusion This study introduces a comprehensive analysis method of chemical constituents in Jiashen Table extract. The results afford a significant guidance for the study of active constituents and quality control research for Jiashen Tablet.
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[基金项目]
国家科技重大专项课题——中医药优势领域的创新中药关键技术开发研究(2017ZX09301005)