[关键词]
[摘要]
目的 对腰痹通胶囊醇提部位进行化学成分研究。方法 采用硅胶柱色谱、反相色谱(DAC)、Sephadex LH-20凝胶柱色谱以及制备型HPLC等方法进行分离和纯化,并结合NMR等波谱学技术对化合物进行结构鉴定。结果 从腰痹通胶囊醇提部位中共分离得到了16个化合物,分别鉴定为紫花前胡内酯(1)、哥伦比亚苷元(2)、阿魏酸(3)、伞形花内酯(4)、佛手酚(5)、花椒毒素(6)、异欧前胡素(7)、滨蒿内酯(8)、(Z)-6-hydroxy-7-methoxydihydroligustilide(9)、4-羟基-3-丁基苯酞(10)、3-(3'-羟基)-丁基苯酞(11)、洋川芎内酯C(12)、洋川芎内酯H(13)、洋川芎内酯I(14)、秦皮定(15)、异秦皮定(16)。结论 化合物1 ~16 均为首次从该复方中分离得到,化合物11 为首次通过分离手段得到的天然产物。
[Key word]
[Abstract]
Objective To investigate the chemical constituents from the ethanol extract of Yaobitong Capsule. Methods The chemical constituents were isolated by repeated silica gel column, dynamic axial compression chromatography, Sephadex LH-20 column, and prep-HPLC. Their structures were identified on the basis of NMR spectral data analysis. Results Sixteen compounds were isolated and identified to be nodakenetin (1), columbianetin (2), ferulic acid (3), umbelliferone (4), bergaptol (5), xanthotoxin (6), isoimperatorin (7), scoparone (8), (Z)-6-hydroxy-7-methoxydihydroligustilide (9), 4-hydroxy-3-butylphthalide (10), 3-(3'-hydroxy)-butylphthalide (11), senkyunolide C (12), senkyunolide H (13), senkyunolide I (14), fraxidin (15), and isofraxidin (16). Conclusion Compounds 1 -16 are isolated from Yaobitong Capsule for the first time, and compound 11 is obtained by the means of separation for the first time.
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[基金项目]
科技部重大新药创制:现代中药创新集群与数字制药技术平台(2013ZX09402203)