目的 对丹参Salvia miltiorrhiza基因组中存在的Pht1基因家族成员进行挖掘、鉴定和功能预测分析，为进一步研究和利用Pht1这一功能基因家族奠定基础。方法 基于丹参基因组数据库及NCBI数据库，借助生物信息学方法，首先筛选获得丹参Pht1家族候选基因的序列，进而对其进行多重序列比对、保守结构查找及系统进化树构建，最后完成其特征性分析和生物学功能预测。结果 从丹参基因组中共挖掘出了12个新的Pht1候选基因，加上已报道的Smpht1，共13个Pht1成员，其同源性高达74.34%，并对9个成员提供了比较可靠的功能注释。该家族基因中，Smpht1&Sm1、Sm4&Sm6、Sm5&Sm11这3对成员可能属于彼此协同进化的同功能基因；Smpht1、Sm1、Sm3、Sm5、Sm7及Sm11这6个成员在丹参根部表达，除了受缺磷特异性诱导表达的Sm7和不受磷素供缺因素所影响的Sm3以外，其余4个均有可能属于缺磷诱导增强表达型基因，进而对丹参磷素吸收起着重要作用；另外，Sm7与Sm16还有可能在丹参花器官中表达而参与植株花期磷素平衡的调节；Sm14则很有可能是一个菌根诱导特异性表达的磷转运基因。结论 首次系统地从丹参基因组中挖掘筛选出Pht1基因家族，并发现了具有特征性生物学功能的成员，这将为进一步阐明Pht1在丹参生长发育和药效成分代谢中所起作用的相关研究提供背景支持和思路参考。

Objective To provide a basis for further study and utilization of the functional gene family of Pht1 in Salviae miltiorrhizae, the Pht1 gene family members from S. miltiorrhizae genome should be explored, identified and functionally predicted. Methods By utilizing the bioinformatics methods, based on the genomic and NCBI database of S. miltiorrhiza, the sequences of Pht1 family candidate genes of S. miltiorrhiza were obtained firstly by screening, and then carried on the multiple sequence alignment, conserved structure lookup as well as phylogenetic tree construction. Finally, the characteristic analysis and biological function prediction were carried out. Results The results showed that 12 Pht1 candidate genes were extracted from the genome of Salvia miltiorrhiza, together with the reported Smpht1, there are 13 Pht1 members in total, their homology as high as 74.34%, and nine members of them could provide more reliable functional annotation. Among the gene family members, Smpht1 & Sm1, Sm4 & Sm6, Sm5 & Sm11, in which these three pairs may share similar function as well as coevolution relation between each other; Smpht1, Sm1, Sm3, Sm5, Sm7, and Sm11, all of the six members can be only expressed in the roots of S. miltiorrhiza. furthermore, except Sm7 induced by p-deficiency and Sm3 member unaffected by the phosphorus supply factor, the other four probably belong to the P-deficiency induced up-regulated expressive genotype, which play an important role in phosphorus absorption of S. miltiorrhiza; Moreover, both Sm7 and Sm16 are possible to expressed in the flower organs of S. miltiorrhiza and then participate in the regulation of phosphorus balance in the flowering stage of plants. In addition, Sm14 is likely to be a mycorrhizal-induced specific expressive gene with the function of phosphorus transfer in Pht1 family members. Conclusion The Pht1 gene family was screened and identified systematically from the genome of S. miltiorrhiza for the first time, in addition, the members with characteristic and biological function were found out, which will provide dependable background support and ideas reference for the further research on the role of Pht1 in the growth and development of S. miltiorrhiza.

国家自然科学基金资助项目（81173493）